Recombinant Anti-FGFR4 antibody [EPR5330(N)] (ab178396)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5330(N)] to FGFR4
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-FGFR4 antibody [EPR5330(N)]
See all FGFR4 primary antibodies -
Description
Rabbit monoclonal [EPR5330(N)] to FGFR4 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- SW480, SH-SY5Y, Raji and MOLT4 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5330(N) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab178396 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 88 kDa.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 88 kDa. |
Target
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Function
Receptor for acidic fibroblast growth factor. Does not bind to basic fibroblast growth factor. Binds FGF19. -
Tissue specificity
Expressed in gastrointestinal epithelial cells, pancreas, and gastric and pancreatic cancer cell lines. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Post-translational
modificationsGlycosylated.
Phosphorylated on tyrosine residue (By similarity). Phosphorylation requires the presence of a functional (phosphorylated) FGFR1 and not necessarily by means of FGFR heterodimerization. -
Cellular localization
Membrane. Isoform 2 may be secreted. - Information by UniProt
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Database links
- Entrez Gene: 2264 Human
- Entrez Gene: 14186 Mouse
- Entrez Gene: 25114 Rat
- Omim: 134935 Human
- SwissProt: P22455 Human
- SwissProt: Q03142 Mouse
- SwissProt: Q498D6 Rat
- Unigene: 165950 Human
see all -
Alternative names
- CD 334 antibody
- CD334 antibody
- CD334 antigen antibody
see all
Images
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All lanes : Anti-FGFR4 antibody [EPR5330(N)] (ab178396) at 1/1000 dilution
Lane 1 : SW480 cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : Raji cell lysate
Lane 4 : MOLT4 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 88 kDaThe expression profile observed is consistent with what has been described in the literature PMID: 15709206.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab178396 has been referenced in 4 publications.
- Dong Z et al. Hepatic Reduction in Cholesterol 25-Hydroxylase Aggravates Diet-induced Steatosis. Cell Mol Gastroenterol Hepatol 13:1161-1179 (2022). PubMed: 34990887
- Zhang D et al. Dexamethasone and lenvatinib inhibit migration and invasion of non-small cell lung cancer by regulating EKR/AKT and VEGF signal pathways. Exp Ther Med 19:762-770 (2020). PubMed: 31853327
- Ji S et al. FGF15 Activates Hippo Signaling to Suppress Bile Acid Metabolism and Liver Tumorigenesis. Dev Cell 48:460-474.e9 (2019). PubMed: 30745141
- Wang J et al. Myeloid Cell-Specific Lipin-1 Deficiency Stimulates Endocrine Adiponectin-FGF15 Axis and Ameliorates Ethanol-Induced Liver Injury in Mice. Sci Rep 6:34117 (2016). WB ; Mouse . PubMed: 27666676