Anti-FH antibody [8F12BB5] (ab110286)


  • Product name
    Anti-FH antibody [8F12BB5]
    See all FH primary antibodies
  • Description
    Mouse monoclonal [8F12BB5] to FH
  • Host species
  • Tested applications
    Suitable for: In-Cell ELISA, IP, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Rat, Cow, Human
  • Immunogen

    Purified FH proteins from bovine liver, bovine heart, porcine heart

  • Positive control
    • Human HDFn cells; Human cerebellum tissue; HepG2 cells, human liver mitochondria, bovine liver mitochondria, and rat liver mitochondria; HeLa cells
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact or you can find further information here.



Our Abpromise guarantee covers the use of ab110286 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
In-Cell ELISA Use a concentration of 4 µg/ml. 0.4 µg/well
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval - 1 min pressure cook in 1mmol EDTA pH8.
ICC/IF Use a concentration of 5 µg/ml.
Flow Cyt Use a concentration of 1 µg/ml.

Recommend 0.1% Triton X-100 permeabilization.




ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.



  • Function
    Also acts as a tumor suppressor.
  • Pathway
    Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1.
  • Involvement in disease
    Defects in FH are the cause of fumarase deficiency (FHD) [MIM:606812]; also known as fumaricaciduria. FHD is characterized by progressive encephalopathy, developmental delay, hypotonia, cerebral atrophy and lactic and pyruvic acidemia.
    Defects in FH are the cause of multiple cutaneous and uterine leiomyomata (MCUL1) [MIM:150800]. MCUL1 is an autosomal dominant condition in which affected individuals develop benign smooth muscle tumors (leiomyomata) of the skin. Affected females also usually develop leiomyomata of the uterus (fibroids).
    Defects in FH are the cause of hereditary leiomyomatosis and renal cell cancer (HLRCC) [MIM:605839].
  • Sequence similarities
    Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.
  • Cellular localization
    Cytoplasm and Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • FH antibody
    • Fumarase antibody
    • Fumarate hydratase antibody
    • Fumarate hydratase mitochondrial antibody
    • Fumarate hydratase, mitochondrial antibody
    • FUMH_HUMAN antibody
    • HLRCC antibody
    • LRCC antibody
    • MCL antibody
    • MCUL 1 antibody
    • MCUL1 antibody
    • MS709 antibody
    • Multiple hereditary cutaneous leiomyomata antibody
    see all


  • HeLa cells were stained with 1 µg/mL ab110286 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
  • Immunocytochemistry image of FH (ab110286) stained human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with ab110286 at 5µg/ml for 2h at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.
  • FH immunocaptured from HepG2 cells (Lane 1), human liver mitochondria (Lane 2), bovine liver mitochondria (Lane 3), and rat liver mitochondria (Lane 4) using ab110286.
  • ab110286 staining FH in formalin-fixed, paraffin-embedded human cerebellum tissue by Immunohistochemistry at 5ug/ml. FH immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells.


ab110286 has not yet been referenced specifically in any publications.

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