Anti-FH antibody (ab95950)
- Datasheet
- References
- Protocols
Overview
-
Product nameAnti-FH antibody
See all FH primary antibodies -
DescriptionRabbit polyclonal to FH
-
Host speciesRabbit
-
Tested applicationsSuitable for: WB, IHC-Pmore details
-
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Zebrafish
-
Immunogen
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 89 and 335 of FH
-
Positive control
- 293T and A431 whole cell lysates OVCAR3 xenograft tissue
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
-
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 1.21% Tris, 0.75% Glycine -
Concentration information loading... -
PurityImmunogen affinity purified
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab95950 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 55 kDa. | |
| IHC-P | 1/100 - 1/500. |
Target
-
FunctionAlso acts as a tumor suppressor.
-
PathwayCarbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1.
-
Involvement in diseaseDefects in FH are the cause of fumarase deficiency (FHD) [MIM:606812]; also known as fumaricaciduria. FHD is characterized by progressive encephalopathy, developmental delay, hypotonia, cerebral atrophy and lactic and pyruvic acidemia.
Defects in FH are the cause of multiple cutaneous and uterine leiomyomata (MCUL1) [MIM:150800]. MCUL1 is an autosomal dominant condition in which affected individuals develop benign smooth muscle tumors (leiomyomata) of the skin. Affected females also usually develop leiomyomata of the uterus (fibroids).
Defects in FH are the cause of hereditary leiomyomatosis and renal cell cancer (HLRCC) [MIM:605839]. -
Sequence similaritiesBelongs to the class-II fumarase/aspartase family. Fumarase subfamily.
-
Cellular localizationCytoplasm and Mitochondrion.
- Information by UniProt
-
Database links
- Entrez Gene: 520260 Cow
- Entrez Gene: 2271 Human
- Entrez Gene: 14194 Mouse
- Entrez Gene: 24368 Rat
- Entrez Gene: 393938 Zebrafish
- Omim: 136850 Human
- SwissProt: P07954 Human
- SwissProt: P97807 Mouse
see all -
Alternative names
- FH antibody
- Fumarase antibody
- Fumarate hydratase antibody
see all
Images
-
Western blot - Anti-FH antibody (ab95950)All lanes : Anti-FH antibody (ab95950) at 1/5000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 55 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FH antibody (ab95950)ab95950 at a 1:500 dilution, staining human FH in OVCAR3 xenograft, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.
References
ab95950 has not yet been referenced specifically in any publications.
