Anti-FH/Fumarase antibody (ab191367)

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Overview

  • Product name

    Anti-FH/Fumarase antibody
    See all FH/Fumarase primary antibodies

  • Description

    Rabbit polyclonal to FH/Fumarase

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human FH/Fumarase aa 89-365. The exact sequence is proprietary.
    Sequence:

    PTPVIKAFGILKRAAAEVNQDYGLDPKIANAIMKAADEVAEGKLNDHFPL VVWQTGSGTQTNMNVNEVISNRAIEMLGGELGSKIPVHPNDHVNKSQSSN DTFPTAMHIAAAIEVHEVLLPGLQKLHDALDAKSKEFAQIIKIGRTHTQD AVPLTLGQEFSGYVQQVKYAMTRIKAAMPRIYELAAGGTAVGTGLNTRIG FAEKVAAKVAALTGLPFVTAPNKFEALAAHDALVELSGAMNTTACSLMKI ANDIRFLGSGPRSGLGELILPENEPGS


    Database link: P07954

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human liver tissue; HeLa cells; rat and mouse liver tissue lysates; 293T and A431 cell lysates.

  • General notes

     This product was previously labelled as FH

     

Properties

Applications

Our Abpromise guarantee covers the use of ab191367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 54 kDa.
IHC-P Use a concentration of 7.5 µg/ml.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Also acts as a tumor suppressor.

  • Pathway

    Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1.

  • Involvement in disease

    Defects in FH are the cause of fumarase deficiency (FHD) [MIM:606812]; also known as fumaricaciduria. FHD is characterized by progressive encephalopathy, developmental delay, hypotonia, cerebral atrophy and lactic and pyruvic acidemia.
    Defects in FH are the cause of multiple cutaneous and uterine leiomyomata (MCUL1) [MIM:150800]. MCUL1 is an autosomal dominant condition in which affected individuals develop benign smooth muscle tumors (leiomyomata) of the skin. Affected females also usually develop leiomyomata of the uterus (fibroids).
    Defects in FH are the cause of hereditary leiomyomatosis and renal cell cancer (HLRCC) [MIM:605839].

  • Sequence similarities

    Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.

  • Cellular localization

    Cytoplasm and Mitochondrion.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • FH antibody
    • Fumarase antibody
    • Fumarate hydratase antibody
    • Fumarate hydratase mitochondrial antibody
    • Fumarate hydratase, mitochondrial antibody
    • FUMH_HUMAN antibody
    • HLRCC antibody
    • LRCC antibody
    • MCL antibody
    • MCUL 1 antibody
    • MCUL1 antibody
    • MS709 antibody
    • Multiple hereditary cutaneous leiomyomata antibody
    see all

Images

  • Western blot - Anti-FH/Fumarase antibody (ab191367)
    Western blot - Anti-FH/Fumarase antibody (ab191367)
    All lanes : Anti-FH/Fumarase antibody (ab191367) at 1/5000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : A431 cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 54 kDa



    10% SDS Page

  • Western blot - Anti-FH/Fumarase antibody (ab191367)
    Western blot - Anti-FH/Fumarase antibody (ab191367)
    Anti-FH/Fumarase antibody (ab191367) at 1/1000 dilution + mouse liver lysate at 50 µg

    Predicted band size: 54 kDa



    10% SDS Page

  • Western blot - Anti-FH/Fumarase antibody (ab191367)
    Western blot - Anti-FH/Fumarase antibody (ab191367)
    Anti-FH/Fumarase antibody (ab191367) at 1/5000 dilution + rat liver tissue lysate at 50 µg

    Predicted band size: 54 kDa



    10% SDS Page

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FH/Fumarase antibody (ab191367)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FH/Fumarase antibody (ab191367)

    Immunohistochemical analysis of formalin fixed, paraffin embedded Human liver tissue labeling FH/Fumarase using ab191367 at 7.5 µg/ml.

  • Immunocytochemistry/ Immunofluorescence - Anti-FH/Fumarase antibody (ab191367)
    Immunocytochemistry/ Immunofluorescence - Anti-FH/Fumarase antibody (ab191367)

    Immunofluorescence analysis of methanol-fixed HeLa cells labeling FH/Fumarase using ab191367 at 1/200 dilution. Bottom image shows Hoechst 33342 counterstain.

References

ab191367 has not yet been referenced specifically in any publications.

Publishing research using ab191367? Please let us know so that we can cite the reference in this datasheet.

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