Key features and details
- Rabbit polyclonal to FH/Fumarase
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-FH/Fumarase antibody
See all FH/Fumarase primary antibodies
DescriptionRabbit polyclonal to FH/Fumarase
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide within Human FH/Fumarase aa 150-200. The exact sequence is proprietary.
Database link: P07954
- IP: HeLa whole cell lysate. WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates.
This product was previously labelled as FH
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226365 was affinity purified using an epitope specific to FH/Fumarase immobilized on solid support.
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab226365 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 50,55 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionAlso acts as a tumor suppressor.
PathwayCarbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1.
Involvement in diseaseDefects in FH are the cause of fumarase deficiency (FHD) [MIM:606812]; also known as fumaricaciduria. FHD is characterized by progressive encephalopathy, developmental delay, hypotonia, cerebral atrophy and lactic and pyruvic acidemia.
Defects in FH are the cause of multiple cutaneous and uterine leiomyomata (MCUL1) [MIM:150800]. MCUL1 is an autosomal dominant condition in which affected individuals develop benign smooth muscle tumors (leiomyomata) of the skin. Affected females also usually develop leiomyomata of the uterus (fibroids).
Defects in FH are the cause of hereditary leiomyomatosis and renal cell cancer (HLRCC) [MIM:605839].
Sequence similaritiesBelongs to the class-II fumarase/aspartase family. Fumarase subfamily.
Cellular localizationCytoplasm and Mitochondrion.
- Information by UniProt
- FH antibody
- Fumarase antibody
- Fumarate hydratase antibody
All lanes : Anti-FH/Fumarase antibody (ab226365) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 50,55 kDa
Exposure time: 3 seconds
Cell lysates were prepared with NETN lysis buffer.
FH/Fumarase was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared uing NETN lysis buffer; 20% of IP loaded) with ab226365 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226365 at 0.1 µg/ml.
Lane 1: ab226365 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226365 has not yet been referenced specifically in any publications.