Anti-FH/Fumarase antibody (ab95947)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-FH/Fumarase antibody
See all FH/Fumarase primary antibodies -
Description
Rabbit polyclonal to FH/Fumarase -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Zebrafish
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Immunogen
Recombinant fragment corresponding to Human FH/Fumarase aa 195-443.
Database link: NP_000134 -
Positive control
- HeLa and HepG2 whole cell lysates; HeLa cells; NCIN87 xenograft; 293T, A431, H1299, Molt, and Raji cell lines
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General notes
This product was previously labelled as FH
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab95947 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 55 kDa. | |
| IHC-P | 1/100 - 1/500. | |
| ICC/IF | 1/100 - 1/200. |
Target
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Function
Also acts as a tumor suppressor. -
Pathway
Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1. -
Involvement in disease
Defects in FH are the cause of fumarase deficiency (FHD) [MIM:606812]; also known as fumaricaciduria. FHD is characterized by progressive encephalopathy, developmental delay, hypotonia, cerebral atrophy and lactic and pyruvic acidemia.
Defects in FH are the cause of multiple cutaneous and uterine leiomyomata (MCUL1) [MIM:150800]. MCUL1 is an autosomal dominant condition in which affected individuals develop benign smooth muscle tumors (leiomyomata) of the skin. Affected females also usually develop leiomyomata of the uterus (fibroids).
Defects in FH are the cause of hereditary leiomyomatosis and renal cell cancer (HLRCC) [MIM:605839]. -
Sequence similarities
Belongs to the class-II fumarase/aspartase family. Fumarase subfamily. -
Cellular localization
Cytoplasm and Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 520260 Cow
- Entrez Gene: 2271 Human
- Entrez Gene: 14194 Mouse
- Entrez Gene: 24368 Rat
- Entrez Gene: 393938 Zebrafish
- Omim: 136850 Human
- SwissProt: P07954 Human
- SwissProt: P97807 Mouse
see all -
Alternative names
- FH antibody
- Fumarase antibody
- Fumarate hydratase antibody
see all
Images
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Western blot - Anti-FH/Fumarase antibody (ab95947)All lanes : Anti-FH/Fumarase antibody (ab95947) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 55 kDa
10% SDS-PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FH/Fumarase antibody (ab95947)Immunohistochemical analysis of paraffin embedded NCIN87 xenograft, using ab95947 at 1/500 dilution. -
Immunocytochemistry/ Immunofluorescence - Anti-FH/Fumarase antibody (ab95947)Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, using ab95947 at 1/200 dilution. Right image is merged with DNA probe.
Protocols
References
ab95947 has not yet been referenced specifically in any publications.
