Product nameAnti-FH/Fumarase antibody [EPR11647(2)(B)] - N-terminal
See all FH/Fumarase primary antibodies
DescriptionRabbit monoclonal [EPR11647(2)(B)] to FH/Fumarase - N-terminal
Tested applicationsSuitable for: ICC/IF, WB, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human FH/Fumarase aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: P07954
- HepG2, HeLa, 293 and A549 cells lysates; HeLa cells.
This product was previously labelled as FH
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab184945 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 49 kDa (predicted molecular weight: 55 kDa).|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionAlso acts as a tumor suppressor.
PathwayCarbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1.
Involvement in diseaseDefects in FH are the cause of fumarase deficiency (FHD) [MIM:606812]; also known as fumaricaciduria. FHD is characterized by progressive encephalopathy, developmental delay, hypotonia, cerebral atrophy and lactic and pyruvic acidemia.
Defects in FH are the cause of multiple cutaneous and uterine leiomyomata (MCUL1) [MIM:150800]. MCUL1 is an autosomal dominant condition in which affected individuals develop benign smooth muscle tumors (leiomyomata) of the skin. Affected females also usually develop leiomyomata of the uterus (fibroids).
Defects in FH are the cause of hereditary leiomyomatosis and renal cell cancer (HLRCC) [MIM:605839].
Sequence similaritiesBelongs to the class-II fumarase/aspartase family. Fumarase subfamily.
Cellular localizationCytoplasm and Mitochondrion.
- Information by UniProt
- FH antibody
- Fumarase antibody
- Fumarate hydratase antibody
All lanes : Anti-FH/Fumarase antibody [EPR11647(2)(B)] - N-terminal (ab184945) at 1/2000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293 cell lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Predicted band size: 55 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Immunofluorescence analysis of, -20 °C acetone-fixed, HeLa cells labeling FH/Fumarase with ab184945 at a 1/100 dilution. As secondary antibody goat anti-rabbit IgG (Alexa Fluor®488) was used at a 1/200. In blue DAPI staining.
Flow cytometry analysis of 2% paraformaldehyde-fixed HeLa cells labeling FH/Fumarase with ab184945 at a 1/160 dilution (red) or negative control rabbit IgG (green). Secondary antibody goat anti-rabbit IgG (FITC) at a 1/150 dilution.
ab184945 has not yet been referenced specifically in any publications.