Recombinant
RabMAb

Recombinant Anti-FH/Fumarase antibody [EPR11648] (ab171948)

Overview

  • Product name

    Anti-FH/Fumarase antibody [EPR11648]
    See all FH/Fumarase primary antibodies
  • Description

    Rabbit monoclonal [EPR11648] to FH/Fumarase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    within Human FH/Fumarase aa 450 to the C-terminus. The exact sequence is proprietary.
    Database link: P07954

  • Positive control

    • HepG2 cells and HepG2, HeLa, 293T and A459 cell lysates,
  • General notes

     This product was previously labelled as FH

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab171948 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 50 kDa.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      Also acts as a tumor suppressor.
    • Pathway

      Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1.
    • Involvement in disease

      Defects in FH are the cause of fumarase deficiency (FHD) [MIM:606812]; also known as fumaricaciduria. FHD is characterized by progressive encephalopathy, developmental delay, hypotonia, cerebral atrophy and lactic and pyruvic acidemia.
      Defects in FH are the cause of multiple cutaneous and uterine leiomyomata (MCUL1) [MIM:150800]. MCUL1 is an autosomal dominant condition in which affected individuals develop benign smooth muscle tumors (leiomyomata) of the skin. Affected females also usually develop leiomyomata of the uterus (fibroids).
      Defects in FH are the cause of hereditary leiomyomatosis and renal cell cancer (HLRCC) [MIM:605839].
    • Sequence similarities

      Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.
    • Cellular localization

      Cytoplasm and Mitochondrion.
    • Information by UniProt
    • Database links

    • Alternative names

      • FH antibody
      • Fumarase antibody
      • Fumarate hydratase antibody
      • Fumarate hydratase mitochondrial antibody
      • Fumarate hydratase, mitochondrial antibody
      • FUMH_HUMAN antibody
      • HLRCC antibody
      • LRCC antibody
      • MCL antibody
      • MCUL 1 antibody
      • MCUL1 antibody
      • MS709 antibody
      • Multiple hereditary cutaneous leiomyomata antibody
      see all

    Images

    • Immunofluorescent analysis of HepG2 cells labeling FH/Fumarase with ab171948 (green) and DAPI nuclear staining (blue).

    • All lanes : Anti-FH/Fumarase antibody [EPR11648] (ab171948) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 50 kDa

    References

    ab171948 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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