Product nameAnti-FH/Fumarase antibody [EPR11648]
See all FH/Fumarase primary antibodies
DescriptionRabbit monoclonal [EPR11648] to FH/Fumarase
Tested applicationsSuitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
within Human FH/Fumarase aa 450 to the C-terminus. The exact sequence is proprietary.
Database link: P07954
- HepG2 cells and HepG2, HeLa, 293T and A459 cell lysates,
Storage instructionsShipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab171948 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 50 kDa.|
|ICC/IF||1/100 - 1/250.|
FunctionAlso acts as a tumor suppressor.
PathwayCarbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1.
Involvement in diseaseDefects in FH are the cause of fumarase deficiency (FHD) [MIM:606812]; also known as fumaricaciduria. FHD is characterized by progressive encephalopathy, developmental delay, hypotonia, cerebral atrophy and lactic and pyruvic acidemia.
Defects in FH are the cause of multiple cutaneous and uterine leiomyomata (MCUL1) [MIM:150800]. MCUL1 is an autosomal dominant condition in which affected individuals develop benign smooth muscle tumors (leiomyomata) of the skin. Affected females also usually develop leiomyomata of the uterus (fibroids).
Defects in FH are the cause of hereditary leiomyomatosis and renal cell cancer (HLRCC) [MIM:605839].
Sequence similaritiesBelongs to the class-II fumarase/aspartase family. Fumarase subfamily.
Cellular localizationCytoplasm and Mitochondrion.
- Information by UniProt
- FH antibody
- Fumarase antibody
- Fumarate hydratase antibody
Immunofluorescent analysis of HepG2 cells labeling FH/Fumarase with ab171948 (green) and DAPI nuclear staining (blue).
All lanes : Anti-FH/Fumarase antibody [EPR11648] (ab171948) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 50 kDa
ab171948 has not yet been referenced specifically in any publications.