Recombinant Anti-FH/Fumarase antibody [EPR11648] - BSA and Azide free (ab249667)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11648] to FH/Fumarase - BSA and Azide free
- Suitable for: ICC, WB
- Reacts with: Rat, Human
Overview
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Product name
Anti-FH/Fumarase antibody [EPR11648] - BSA and Azide free
See all FH/Fumarase primary antibodies -
Description
Rabbit monoclonal [EPR11648] to FH/Fumarase - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
Application Species WB Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab249667 is the carrier-free version of ab171948 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab249667 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as FH
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR11648 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab249667 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
WB |
Human
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All applications |
Mouse
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Application | Abreviews | Notes |
---|---|---|
ICC |
Use at an assay dependent concentration.
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|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 50 kDa.
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Notes |
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ICC
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 50 kDa. |
Target
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Function
Also acts as a tumor suppressor. -
Pathway
Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1. -
Involvement in disease
Defects in FH are the cause of fumarase deficiency (FHD) [MIM:606812]; also known as fumaricaciduria. FHD is characterized by progressive encephalopathy, developmental delay, hypotonia, cerebral atrophy and lactic and pyruvic acidemia.
Defects in FH are the cause of multiple cutaneous and uterine leiomyomata (MCUL1) [MIM:150800]. MCUL1 is an autosomal dominant condition in which affected individuals develop benign smooth muscle tumors (leiomyomata) of the skin. Affected females also usually develop leiomyomata of the uterus (fibroids).
Defects in FH are the cause of hereditary leiomyomatosis and renal cell cancer (HLRCC) [MIM:605839]. -
Sequence similarities
Belongs to the class-II fumarase/aspartase family. Fumarase subfamily. -
Cellular localization
Cytoplasm and Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 2271 Human
- Entrez Gene: 14194 Mouse
- Entrez Gene: 24368 Rat
- Omim: 136850 Human
- SwissProt: P07954 Human
- SwissProt: P97807 Mouse
- SwissProt: P14408 Rat
- Unigene: 592490 Human
see all -
Alternative names
- FH antibody
- Fumarase antibody
- Fumarate hydratase antibody
see all
Images
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All lanes : Anti-FH/Fumarase antibody [EPR11648] (ab171948) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 50 kDaThis data was developed using ab171948, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab249667 has not yet been referenced specifically in any publications.