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RecombinantRabMAb

Recombinant Anti-FH/Fumarase antibody [EPR11648] - BSA and Azide free (ab249667)

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  • SDS
  • Certificate of Compliance
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Immunocytochemistry - Anti-FH/Fumarase antibody [EPR11648] - BSA and Azide free (ab249667)
  • Western blot - Anti-FH/Fumarase antibody [EPR11648] - BSA and Azide free (ab249667)
  • Anti-FH/Fumarase antibody [EPR11648] - BSA and Azide free (ab249667)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11648] to FH/Fumarase - BSA and Azide free
  • Suitable for: ICC, WB
  • Reacts with: Rat, Human

You may also be interested in

Protein
Product image
Recombinant human FH/Fumarase protein (ab168013)
Conjugation
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Alexa Fluor® 488 Conjugation Kit (Fast) - Lightning-Link® (ab236553)

View more associated products

Overview

  • Product name

    Anti-FH/Fumarase antibody [EPR11648] - BSA and Azide free
    See all FH/Fumarase primary antibodies
  • Description

    Rabbit monoclonal [EPR11648] to FH/Fumarase - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab249667 is the carrier-free version of ab171948 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab249667 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as FH

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR11648
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Other
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy

Associated products

  • Alternative Versions

    • Anti-FH/Fumarase antibody [EPR11648] (ab171948)
  • Conjugation kits

    • FITC Conjugation Kit (Fast) - Lightning-Link® (ab188285)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • A549 whole cell lysate (ab7910)
  • Recombinant Protein

    • Recombinant human FH/Fumarase protein (ab168013)
  • Related Products

    • Recombinant Human FH/Fumarase protein (ab82790)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab249667 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
All applications
Mouse
Application Abreviews Notes
ICC
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 50 kDa.
Notes
ICC
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 50 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      Also acts as a tumor suppressor.
    • Pathway

      Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1.
    • Involvement in disease

      Defects in FH are the cause of fumarase deficiency (FHD) [MIM:606812]; also known as fumaricaciduria. FHD is characterized by progressive encephalopathy, developmental delay, hypotonia, cerebral atrophy and lactic and pyruvic acidemia.
      Defects in FH are the cause of multiple cutaneous and uterine leiomyomata (MCUL1) [MIM:150800]. MCUL1 is an autosomal dominant condition in which affected individuals develop benign smooth muscle tumors (leiomyomata) of the skin. Affected females also usually develop leiomyomata of the uterus (fibroids).
      Defects in FH are the cause of hereditary leiomyomatosis and renal cell cancer (HLRCC) [MIM:605839].
    • Sequence similarities

      Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.
    • Cellular localization

      Cytoplasm and Mitochondrion.
    • Target information above from: UniProt accession P07954 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 2271 Human
      • Entrez Gene: 14194 Mouse
      • Entrez Gene: 24368 Rat
      • Omim: 136850 Human
      • SwissProt: P07954 Human
      • SwissProt: P97807 Mouse
      • SwissProt: P14408 Rat
      • Unigene: 592490 Human
      • Unigene: 41502 Mouse
      • Unigene: 29782 Rat
      see all
    • Alternative names

      • FH antibody
      • Fumarase antibody
      • Fumarate hydratase antibody
      • Fumarate hydratase mitochondrial antibody
      • Fumarate hydratase, mitochondrial antibody
      • FUMH_HUMAN antibody
      • HLRCC antibody
      • LRCC antibody
      • MCL antibody
      • MCUL 1 antibody
      • MCUL1 antibody
      • MS709 antibody
      • Multiple hereditary cutaneous leiomyomata antibody
      see all

    Images

    • Immunocytochemistry - Anti-FH/Fumarase antibody [EPR11648] - BSA and Azide free (ab249667)
      Immunocytochemistry - Anti-FH/Fumarase antibody [EPR11648] - BSA and Azide free (ab249667)
      This data was developed using ab171948, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HepG2 cells labeling FH/Fumarase with ab171948 (green) and DAPI nuclear staining (blue).
    • Western blot - Anti-FH/Fumarase antibody [EPR11648] - BSA and Azide free (ab249667)
      Western blot - Anti-FH/Fumarase antibody [EPR11648] - BSA and Azide free (ab249667)
      All lanes : Anti-FH/Fumarase antibody [EPR11648] (ab171948) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 50 kDa



      This data was developed using ab171948, the same antibody clone in a different buffer formulation.

       

    • Anti-FH/Fumarase antibody [EPR11648] - BSA and Azide free (ab249667)
      Anti-FH/Fumarase antibody [EPR11648] - BSA and Azide free (ab249667)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab249667? Please let us know so that we can cite the reference in this datasheet.

    ab249667 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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