Anti-FHIT antibody - C-terminal (ab181004)
Key features and details
- Rabbit polyclonal to FHIT - C-terminal
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FHIT antibody - C-terminal
See all FHIT primary antibodies -
Description
Rabbit polyclonal to FHIT - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human FHIT (C terminal). Peptide corresponds to 18 amino acids.
Database link: NP_002003 -
Positive control
- HeLa cells and cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181004 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 5 µg/ml.
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 17 kDa.
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Notes |
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ICC/IF
Use a concentration of 5 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 17 kDa. |
Target
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Relevance
FHIT (fragile histidine triad) cleaves adenosine 5' PPP 5' A to yield AMP and ADP. Alterations and deletions of the FHIT gene are strongly linked to the genesis and establishment of human tumors of the lung, cervix, breast, colon, stomach and pancreas. In normal cells, FHIT may act as a tumor suppressor. FHIT physically associates with ubiquitin conjugating enzyme 9. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 2272 Human
- Omim: 601153 Human
- SwissProt: P49789 Human
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Alternative names
- AP3A hydrolase antibody
- AP3A hydrolase fragile site 3p14.2 antibody
- AP3Aase antibody
see all
Images
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Lane 1 : Anti-FHIT antibody - C-terminal (ab181004) at 1 µg
Lane 2 : Anti-FHIT antibody - C-terminal (ab181004) at 2 µg
All lanes : HeLa cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 17 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted? -
Immunofluorescence analysis of HeLa cells, labeling FHIT using ab181004 at 5 µg/ml.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab181004 has not yet been referenced specifically in any publications.