Key features and details
- Rabbit polyclonal to FHIT - C-terminal
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-FHIT antibody - C-terminal
See all FHIT primary antibodies
DescriptionRabbit polyclonal to FHIT - C-terminal
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Human FHIT (C terminal). Peptide corresponds to 18 amino acids.
Database link: NP_002003
- HeLa cells and cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab181004 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 17 kDa.|
RelevanceFHIT (fragile histidine triad) cleaves adenosine 5' PPP 5' A to yield AMP and ADP. Alterations and deletions of the FHIT gene are strongly linked to the genesis and establishment of human tumors of the lung, cervix, breast, colon, stomach and pancreas. In normal cells, FHIT may act as a tumor suppressor. FHIT physically associates with ubiquitin conjugating enzyme 9.
- AP3A hydrolase antibody
- AP3A hydrolase fragile site 3p14.2 antibody
- AP3Aase antibody
Lane 1 : Anti-FHIT antibody - C-terminal (ab181004) at 1 µg
Lane 2 : Anti-FHIT antibody - C-terminal (ab181004) at 2 µg
All lanes : HeLa cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 17 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?
Immunofluorescence analysis of HeLa cells, labeling FHIT using ab181004 at 5 µg/ml.
ab181004 has not yet been referenced specifically in any publications.