• Product name
  • Description
    Goat polyclonal to FHL1
  • Host species
  • Specificity
    ab26072 is specific for SLIMMER / FHL1 isoform B.
  • Tested applications
    Suitable for: ELISA, WB, ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Human, Pig
  • Immunogen

    Synthetic peptide:


    , corresponding to amino acids 233 - 246 of Human FHL1

  • Positive control
    • Human Heart lysate


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.05% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab26072 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    ELISA: 1/128000.
    ICC/IF: Use at an assay dependent dilution (PMID 19643733).
    IHC-Fr: Use at an assay dependent dilution (PMID 19643733).
    WB: Use at a concentration of 0.01 - 0.1 µg/ml. Predicted molecular weight: 36 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      May have an involvement in muscle development or hypertrophy.
    • Tissue specificity
      Isoform 1 is highly expressed in skeletal muscle and to a lesser extent in heart, placenta, ovary, prostate, testis, small intestine, colon and spleen. Expression is barely detectable in brain, lung, liver, kidney, pancreas, thymus and peripheral blood leukocytes. Isoform 2 is expressed in brain, skeletal muscle and to a lesser extent in heart, colon, prostate and small intestine. Isoform 3 is expressed in testis, heart and skeletal muscle.
    • Involvement in disease
      Defects in FHL1 are the cause of X-linked dominant scapuloperoneal myopathy (SPM) [MIM:300695]. Scapuloperoneal syndrome (SPS) was initially described more than 120 years ago by Jules Broussard as 'une forme hereditaire d'atrophie musculaire progressive' beginning in the lower legs and affecting the shoulder region earlier and more severely than distal arm. The etiology of this condition remains unclear.
      Defects in FHL1 are the cause of X-linked myopathy with postural muscle atrophy (XMPMA) [MIM:300696]. Myopathies are inherited muscle disorders characterized by weakness and atrophy of voluntary skeletal muscle, and several types of myopathy also show involvement of cardiac muscle. XMPMA is a distinct form of adult-onset X-linked recessive myopathy with several features in common with other myopathies, but the presentation of a pseudoathletic phenotype, scapuloperoneal weakness, and bent spine is unique and might render the clinical phenotype distinguishable from other myopathies.
      Defects in FHL1 are the cause of X-linked severe early-onset reducing body myopathy (RBM) [MIM:300717]. RBM is a rare muscle disorder causing progressive muscular weakness and characteristic intracytoplasmic inclusions in myofibers. Clinical presentations of RBM have ranged from early onset fatal to childhood onset to adult onset cases.
      Defects in FHL1 are the cause of X-linked childhood-onset reducing body myopathy (CO-RBM) [MIM:300718]. This disorder is allelic to severe early-onset reducing body myopathy (RBM) [MIM:300717].
    • Sequence similarities
      Contains 3 LIM zinc-binding domains.
    • Developmental stage
      Elevated levels during postnatal muscle growth.
    • Cellular localization
      Cytoplasm; Cytoplasm. Nucleus and Nucleus. Cytoplasm > cytosol. Predominantly nuclear in myoblasts but is cytosolic in differentiated myotubes.
    • Information by UniProt
    • Database links
    • Alternative names
      • bA535K18.1 antibody
      • FHL 1 antibody
      • FHL 1B antibody
      • FHL-1 antibody
      • FHL1 antibody
      • FHL1 protein antibody
      • FHL1_HUMAN antibody
      • FHL1A antibody
      • FHL1B antibody
      • FLH1A antibody
      • Four and a half LIM domains 1 antibody
      • Four and a half LIM domains protein 1 antibody
      • Four and a half Lin11 Isl 1 and Mec 3 domains 1 antibody
      • four-and-a-half Lin11 antibody
      • Isl-1 and Mec-3 antibody
      • KYO T antibody
      • KYOT, mouse, homolog of antibody
      • LIM protein SLIMMER antibody
      • MGC111107 antibody
      • RAM14-1 antibody
      • RBMX1A antibody
      • RBMX1B antibody
      • RBP associated molecule 14-1 antibody
      • RP11-535K18.1 antibody
      • Skeletal muscle LIM protein 1 antibody
      • Skeletal muscle LIM-protein 1 antibody
      • SLIM 1 antibody
      • SLIM antibody
      • SLIM-1 antibody
      • SLIM1 antibody
      • SLIMMER antibody
      • XMPMA antibody
      see all


    • Anti-FHL1 antibody (ab26072) at 0.1 µg/ml
      Predicted band size: 36 kDa

      Exposure time: 1 hour

      ab26072 staining Human Heart lysate (35µg protein in RIPA buffer) by Western Blot. Detected by chemiluminescence.


    This product has been referenced in:
    • Cottle DL  et al. SLIMMER (FHL1B/KyoT3) interacts with the proapoptotic protein Siva-1 (CD27BP) and delays skeletal myoblast apoptosis. J Biol Chem 284:26964-77 (2009). WB, ICC/IF, IHC-Fr ; Mouse . Read more (PubMed: 19643733) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A


    Thank you for contacting us.

    I am sorry to hear you have been experiencing problems with ab26072. The quality of our products is important to us and I would like to reassure you that we investigate all customer complaints.

    In order to monitor the product’s quality I would like to check which the previous lot number that worked perfectly was.

    Have you used the same samples, conditions, incubating times and blocking agents? Have you checked the secondary antibody worked fine?

    I appreciate if you could provide further details. As the antibody is covered by the Abpromise guarantee I will be happy to replace it or refund it.

    Thank you for your cooperation. I look forward to receiving your reply. Have a nice day.

    Read More
    Western blot
    Mouse Cell lysate - other (C2C12 myoblasts)
    Loading amount
    100 µg
    C2C12 myoblasts
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

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    Verified customer

    Submitted Nov 26 2007


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