• Product name

    Anti-FHL1 antibody [EPR7520]
    See all FHL1 primary antibodies
  • Description

    Rabbit monoclonal [EPR7520] to FHL1
  • Host species

  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in the Human FHL1 protein (UniProt Q13642).

  • Positive control

    • Human fetal muscle lysate; Human colon and muscle tissues
  • General notes



    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab133661 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 32 kDa (predicted molecular weight: 36 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function

      May have an involvement in muscle development or hypertrophy.
    • Tissue specificity

      Isoform 1 is highly expressed in skeletal muscle and to a lesser extent in heart, placenta, ovary, prostate, testis, small intestine, colon and spleen. Expression is barely detectable in brain, lung, liver, kidney, pancreas, thymus and peripheral blood leukocytes. Isoform 2 is expressed in brain, skeletal muscle and to a lesser extent in heart, colon, prostate and small intestine. Isoform 3 is expressed in testis, heart and skeletal muscle.
    • Involvement in disease

      Defects in FHL1 are the cause of X-linked dominant scapuloperoneal myopathy (SPM) [MIM:300695]. Scapuloperoneal syndrome (SPS) was initially described more than 120 years ago by Jules Broussard as 'une forme hereditaire d'atrophie musculaire progressive' beginning in the lower legs and affecting the shoulder region earlier and more severely than distal arm. The etiology of this condition remains unclear.
      Defects in FHL1 are the cause of X-linked myopathy with postural muscle atrophy (XMPMA) [MIM:300696]. Myopathies are inherited muscle disorders characterized by weakness and atrophy of voluntary skeletal muscle, and several types of myopathy also show involvement of cardiac muscle. XMPMA is a distinct form of adult-onset X-linked recessive myopathy with several features in common with other myopathies, but the presentation of a pseudoathletic phenotype, scapuloperoneal weakness, and bent spine is unique and might render the clinical phenotype distinguishable from other myopathies.
      Defects in FHL1 are the cause of X-linked severe early-onset reducing body myopathy (RBM) [MIM:300717]. RBM is a rare muscle disorder causing progressive muscular weakness and characteristic intracytoplasmic inclusions in myofibers. Clinical presentations of RBM have ranged from early onset fatal to childhood onset to adult onset cases.
      Defects in FHL1 are the cause of X-linked childhood-onset reducing body myopathy (CO-RBM) [MIM:300718]. This disorder is allelic to severe early-onset reducing body myopathy (RBM) [MIM:300717].
    • Sequence similarities

      Contains 3 LIM zinc-binding domains.
    • Developmental stage

      Elevated levels during postnatal muscle growth.
    • Cellular localization

      Cytoplasm; Cytoplasm. Nucleus and Nucleus. Cytoplasm > cytosol. Predominantly nuclear in myoblasts but is cytosolic in differentiated myotubes.
    • Information by UniProt
    • Database links

    • Alternative names

      • bA535K18.1 antibody
      • FHL 1 antibody
      • FHL 1B antibody
      • FHL-1 antibody
      • FHL1 antibody
      • FHL1 protein antibody
      • FHL1_HUMAN antibody
      • FHL1A antibody
      • FHL1B antibody
      • FLH1A antibody
      • Four and a half LIM domains 1 antibody
      • Four and a half LIM domains protein 1 antibody
      • Four and a half Lin11 Isl 1 and Mec 3 domains 1 antibody
      • four-and-a-half Lin11 antibody
      • Isl-1 and Mec-3 antibody
      • KYO T antibody
      • KYOT, mouse, homolog of antibody
      • LIM protein SLIMMER antibody
      • MGC111107 antibody
      • RAM14-1 antibody
      • RBMX1A antibody
      • RBMX1B antibody
      • RBP associated molecule 14-1 antibody
      • RP11-535K18.1 antibody
      • Skeletal muscle LIM protein 1 antibody
      • Skeletal muscle LIM-protein 1 antibody
      • SLIM 1 antibody
      • SLIM antibody
      • SLIM-1 antibody
      • SLIM1 antibody
      • SLIMMER antibody
      • XMPMA antibody
      see all


    • Anti-FHL1 antibody [EPR7520] (ab133661) at 1/1000 dilution + Human fetal muscle lysate at 10 µg

      HRP labelled Goat anti-Rabbit at 1/2000 dilution

      Predicted band size: 36 kDa
      Observed band size: 32 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemistry analysis of FHL1 in paraffin embedded Human colon tissue labelled with ab133661 at a 1/100 dilution.
    • Immunohistochemistry analysis of FHL1 in paraffin embedded Human muscle tissue labelled with ab133661 at a 1/100 dilution.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD


    This product has been referenced in:

    • Du Y  et al. Effects of FHL1 and P21 on hypoxia-induced pulmonary vascular remodeling in neonatal rats. Exp Ther Med 14:4245-4253 (2017). Read more (PubMed: 29067108) »
    • Hemming S  et al. Identification of Novel EZH2 Targets Regulating Osteogenic Differentiation in Mesenchymal Stem Cells. Stem Cells Dev 25:909-21 (2016). Read more (PubMed: 27168161) »
    See all 2 Publications for this product

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