Overview

  • Product name

  • Description

    Rabbit polyclonal to FHL2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human, Pig
    Predicted to work with: Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 52-272 of Human FHL2 (UniProt ID: Q14192).

  • Positive control

    • HeLa whole cell lysate (ab150035); mouse brain whole cell lysate; DLD1 xenograft. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab137477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/3000. Predicted molecular weight: 32 kDa.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • Function

    May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth.
  • Tissue specificity

    Expressed in skeletal muscle and heart.
  • Sequence similarities

    Contains 4 LIM zinc-binding domains.
  • Domain

    The third LIM zinc-binding mediates interaction with E4F1.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • AAG 11 antibody
    • AAG11 antibody
    • Aging associated gene 11 antibody
    • Down regulated in rhabdomyosarcoma LIM protein antibody
    • Downregulated in rhabdomyosarcoma LIM protein antibody
    • DRAL antibody
    • FHL 2 antibody
    • FHL-2 antibody
    • Fhl2 antibody
    • FHL2 protein antibody
    • FHL2_HUMAN antibody
    • Four and a half LIM domain protein 2 antibody
    • Four and a half LIM domains 2 antibody
    • Four and a half LIM domains protein 2 antibody
    • KIAA0990 antibody
    • LIM domain protein DRAL antibody
    • Skeletal muscle LIM protein 3 antibody
    • Skeletal muscle LIM-protein 3 antibody
    • SLIM 3 antibody
    • SLIM-3 antibody
    • SLIM3 antibody
    see all

Images

  • Anti-FHL2 antibody (ab137477) at 1/1500 dilution + Pig aortic valve interstitial cells lysate at 2.5 µg

    Secondary
    HRP-conjugated Goat anti-rabbit IgG (H+L) polyclonal at 1/4500 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 32 kDa
    Observed band size: 32 kDa


    Exposure time: 5 minutes

    See Abreview

  • ICC/IF image of ab137477 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab137477 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-FHL2 antibody (ab137477) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 32 kDa



    12% SDS PAGE
  • Anti-FHL2 antibody (ab137477) at 1/1000 dilution + mouse brain whole cell lysate at 50 µg

    Predicted band size: 32 kDa



    12% SDS PAGE
  • Immunohistochemical analysis of paraffin embedded DLD1 xenograft labelling FHL2 with ab137477 at a 1/100 dilution.

References

ab137477 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human pluripotent stem cell derived cardiomyocyte)
Specification
Human pluripotent stem cell derived cardiomyocyte
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Apr 21 2015

Application
Western blot
Loading amount
2.5 µg
Gel Running Conditions
Reduced Denaturing (8% Tris-Glycine)
Sample
Pig Cell lysate - nuclear (Aortic Valve Interstitial Cells)
Specification
Aortic Valve Interstitial Cells
Blocking step
BSA as blocking agent for 1 minute(s) · Concentration: 1% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Aug 09 2013

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