Anti-FHL2 antibody (ab66399)

Submit a review Q&A (2)References (3)

Overview

  • Product name

    Anti-FHL2 antibody
    See all FHL2 primary antibodies

  • Description

    Rabbit polyclonal to FHL2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish

  • Immunogen

    Synthetic peptide: YAKKCAGCTN PISGLGGTKY ISFEERQWHN DCFNCKKCSL SLVGRGFLTE, corresponding to a region within C terminal amino acids 217-266 of Human FHL2

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Myocardium

Properties

Applications

Our Abpromise guarantee covers the use of ab66399 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.6 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 32 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • Function

    May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth.

  • Tissue specificity

    Expressed in skeletal muscle and heart.

  • Sequence similarities

    Contains 4 LIM zinc-binding domains.

  • Domain

    The third LIM zinc-binding mediates interaction with E4F1.

  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • AAG 11 antibody
    • AAG11 antibody
    • Aging associated gene 11 antibody
    • Down regulated in rhabdomyosarcoma LIM protein antibody
    • Downregulated in rhabdomyosarcoma LIM protein antibody
    • DRAL antibody
    • FHL 2 antibody
    • FHL-2 antibody
    • Fhl2 antibody
    • FHL2 protein antibody
    • FHL2_HUMAN antibody
    • Four and a half LIM domain protein 2 antibody
    • Four and a half LIM domains 2 antibody
    • Four and a half LIM domains protein 2 antibody
    • KIAA0990 antibody
    • LIM domain protein DRAL antibody
    • Skeletal muscle LIM protein 3 antibody
    • Skeletal muscle LIM-protein 3 antibody
    • SLIM 3 antibody
    • SLIM-3 antibody
    • SLIM3 antibody
    see all

Images

  • Western blot - Anti-FHL2 antibody (ab66399)
    Western blot - Anti-FHL2 antibody (ab66399)
    Anti-FHL2 antibody (ab66399) at 0.6 µg/ml + Fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 32 kDa
    Observed band size: 32 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL2 antibody (ab66399)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL2 antibody (ab66399)
    ab66399, at 8µg/ml, staining Human FHL2 in myocardial cells (indicated with arrows) in paraffin-embedded heart tissue, by Immunohistochemistry, 400x magnification.

References

This product has been referenced in:

  • Verset L  et al. Epithelial expression of FHL2 is negatively associated with metastasis-free and overall survival in colorectal cancer. Br J Cancer 109:114-20 (2013). IHC . Read more (PubMed: 23756870) »
  • Matulis CK & Mayo KE The LIM domain protein FHL2 interacts with the NR5A family of nuclear receptors and CREB to activate the inhibin-a subunit gene in ovarian granulosa cells. Mol Endocrinol 26:1278-90 (2012). Human . Read more (PubMed: 22734036) »
See all 3 Publications for this product

Publishing research using ab66399? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-2 of 2 Q&A

Question

protocol IHC-P for ab66399

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Answer

Merci de nous avoir contactés.

Le laboratoire qui produit l'anticorps ab66399 m'a confirmé que le démasquage de l'antigène en IHC-P a été effectuéà 98-100°C, en micro-ondes,avec un tampon0.01 M citrate (pH 6.0)pendant 15-20 minutes. Les lames ont été ensuite laissées à refroidirpendant 30-60 minutes.

La publication d'Emoto dont je vous ai parlé au téléphone est:Emoto et al., J Histochem Cytochem. 2005 Nov ;53 (11) : 1311-21.

J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

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Question

Dear Mr/Mrs: Thanks for your attention. I have ordered two primary antibodies ( Rabbit polyclonal to FHL2.  ab66399 and goat antibody Runx2, ab102712), the illustration shows that it can be used in IHC-P and WB. I wrote this letter to ask whether the primary antibody can be used in IF. I look forword for your early reply. Sincerely yours      

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Answer

Thank you for contacting us and for your interest in our products. If you want to use the antibody for immunofluorescence on cell culture samples (ICC-IF, immunocytochemistry), I am sorry to confirm that as far as we are aware, ab66399 and ab102712 have not been tested and guaranteed in ICC-IF. All tested applications are specified on our datasheets, and these are updated as soon as any new information is brought to our attention. I can confirm that both these antibodies are tested in paraffin embedded sections (IHC-P), we can guarantee this application whether a fluorescent or enzymatic conjugate is used. ab102712 has been tested in Frozen sections (IHC-Fr), and again we can guarantee this application for this antibody whether a fluorescent or enzymatic conjugate is used. The other antibody ab66399 has not been tested and guarnateed for this application. We like to encourage all of our customers to submit an Abreview via the online product datasheet, particularly if they are testing in a new application. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates. To find out more about our Abreview system, please see the following link: https://www.abcam.com/abreviews I hope this information is helpful. Please do not hesitate to contact me for any further advice or information.

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