Overview

  • Product name

    Anti-FHL2 antibody - C-terminal
    See all FHL2 primary antibodies
  • Description

    Rabbit polyclonal to FHL2 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide corresponding to a region within C-terminal amino acids 217-279 of Human FHL2 (Q14192).

  • Positive control

    • A431, HeLa, HepG2, MOLT4, Raji whole cell lysates, Human colon carcinoma tissue, HeLa cells

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab153816 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 32 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Function

    May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth.
  • Tissue specificity

    Expressed in skeletal muscle and heart.
  • Sequence similarities

    Contains 4 LIM zinc-binding domains.
  • Domain

    The third LIM zinc-binding mediates interaction with E4F1.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • AAG 11 antibody
    • AAG11 antibody
    • Aging associated gene 11 antibody
    • Down regulated in rhabdomyosarcoma LIM protein antibody
    • Downregulated in rhabdomyosarcoma LIM protein antibody
    • DRAL antibody
    • FHL 2 antibody
    • FHL-2 antibody
    • Fhl2 antibody
    • FHL2 protein antibody
    • FHL2_HUMAN antibody
    • Four and a half LIM domain protein 2 antibody
    • Four and a half LIM domains 2 antibody
    • Four and a half LIM domains protein 2 antibody
    • KIAA0990 antibody
    • LIM domain protein DRAL antibody
    • Skeletal muscle LIM protein 3 antibody
    • Skeletal muscle LIM-protein 3 antibody
    • SLIM 3 antibody
    • SLIM-3 antibody
    • SLIM3 antibody
    see all

Images

  • Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling FHL2 with ab153816 at 1/200 dilution. Image at the bottom merged with DNA probe.
  • Anti-FHL2 antibody - C-terminal (ab153816) at 1/1000 dilution + HepG2 whole cell
    lysate at 30 µg

    Predicted band size: 32 kDa



    12% SDS PAGE
  • Immunohistochemical analysis of paraffin embedded Human colon carcinoma tissue labeling FHL2 with ab153816 antibody at 1/500.

References

ab153816 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Sample
Human Cell (Human embryonic stem cell derived cardiomyocytes)
Specification
Human embryonic stem cell derived cardiomyocytes
Permeabilization
Yes - Triton
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Dec 12 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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