Overview

  • Product name

  • Description

    Rabbit polyclonal to FHOD1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Sheep, Cow, Cat, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human FHOD1 aa 1114-1164. The exact sequence is proprietary.
    Sequence:

    SVTKSSPRALAARERKRSRGNRKSLRRTLKSGLGDDLVQALGLSKGPGLE V


    Database link: Q9Y613

  • Positive control

    • HeLa and 293T whole cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab206692 was affinity purified using an epitope specific to FHOD1 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab206692 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 127 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Required for the assembly of F-actin structures, such as stress fibers. Depends on the Rho-ROCK cascade for its activity. Contributes to the coordination of microtubules with actin fibers and plays a role in cell elongation.
  • Tissue specificity

    Ubiquitous. Highly expressed in spleen.
  • Sequence similarities

    Belongs to the formin homology family.
    Contains 1 FH1 (formin homology 1) domain.
    Contains 1 FH2 (formin homology 2) domain.
    Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.
  • Domain

    Regulated by intramolecular binding to a C-terminal auto-inhibitory domain. Effector binding abolishes this interaction and activates the protein.
  • Cellular localization

    Cytoplasm. Cytoplasm > cytoskeleton. Predominantly cytoplasmic.
  • Information by UniProt
  • Database links

  • Alternative names

    • FH1/FH2 domain containing protein antibody
    • FH1/FH2 domain containing protein 1 antibody
    • FH1/FH2 domain-containing protein 1 antibody
    • Fhod1 antibody
    • FHOD1_HUMAN antibody
    • FHOS antibody
    • FHOS1 antibody
    • Formin homolog overexpressed in spleen 1 antibody
    • Formin homology 2 domain containing 1 antibody
    • Formin homology 2 domain containing protein 1 antibody
    • Formin homology 2 domain-containing protein 1 antibody
    see all

Images

  • All lanes : Anti-FHOD1 antibody (ab206692) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : Jurkat whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 127 kDa


    Exposure time: 3 minutes


    Lysates prepared using NETN lysis buffer.

  • Detection of FHOD1 in immunoprecipitates of 293T whole cell lysates prepared using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab206692 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 3 minutes.

References

ab206692 has not yet been referenced specifically in any publications.

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