Key features and details
- Rabbit polyclonal to FHOD1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-FHOD1 antibody
See all FHOD1 primary antibodies
DescriptionRabbit polyclonal to FHOD1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Sheep, Cow, Cat, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
- HeLa and 293T whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab206692 was affinity purified using an epitope specific to FHOD1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab206692 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 127 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionRequired for the assembly of F-actin structures, such as stress fibers. Depends on the Rho-ROCK cascade for its activity. Contributes to the coordination of microtubules with actin fibers and plays a role in cell elongation.
Tissue specificityUbiquitous. Highly expressed in spleen.
Sequence similaritiesBelongs to the formin homology family.
Contains 1 FH1 (formin homology 1) domain.
Contains 1 FH2 (formin homology 2) domain.
Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.
DomainRegulated by intramolecular binding to a C-terminal auto-inhibitory domain. Effector binding abolishes this interaction and activates the protein.
Cellular localizationCytoplasm. Cytoplasm > cytoskeleton. Predominantly cytoplasmic.
- Information by UniProt
- FH1/FH2 domain containing protein antibody
- FH1/FH2 domain containing protein 1 antibody
- FH1/FH2 domain-containing protein 1 antibody
All lanes : Anti-FHOD1 antibody (ab206692) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 127 kDa
Exposure time: 3 minutes
Lysates prepared using NETN lysis buffer.
Detection of FHOD1 in immunoprecipitates of 293T whole cell lysates prepared using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab206692 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 3 minutes.
ab206692 has not yet been referenced specifically in any publications.