Anti-FHOD1 antibody (ab73443)
Key features and details
- Mouse polyclonal to FHOD1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FHOD1 antibody
See all FHOD1 primary antibodies -
Description
Mouse polyclonal to FHOD1 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human FHOD1. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- K-562 cell lysate and FHOD1 transfected 293T cell lysate human spleen
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab73443 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 127 kDa.
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IHC-P |
Use a concentration of 13 µg/ml.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 127 kDa. |
IHC-P
Use a concentration of 13 µg/ml. |
Target
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Function
Required for the assembly of F-actin structures, such as stress fibers. Depends on the Rho-ROCK cascade for its activity. Contributes to the coordination of microtubules with actin fibers and plays a role in cell elongation. -
Tissue specificity
Ubiquitous. Highly expressed in spleen. -
Sequence similarities
Belongs to the formin homology family.
Contains 1 FH1 (formin homology 1) domain.
Contains 1 FH2 (formin homology 2) domain.
Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain. -
Domain
Regulated by intramolecular binding to a C-terminal auto-inhibitory domain. Effector binding abolishes this interaction and activates the protein. -
Cellular localization
Cytoplasm. Cytoplasm > cytoskeleton. Predominantly cytoplasmic. - Information by UniProt
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Database links
- Entrez Gene: 29109 Human
- Omim: 606881 Human
- SwissProt: Q9Y613 Human
- Unigene: 95231 Human
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Alternative names
- FH1/FH2 domain containing protein antibody
- FH1/FH2 domain containing protein 1 antibody
- FH1/FH2 domain-containing protein 1 antibody
see all
Images
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All lanes : Anti-FHOD1 antibody (ab73443) at 1/500 dilution
Lane 1 : FHOD1 transfected 293T cell lysate
Lane 2 : non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : goat anti-mouse IgG(H&L)-HRP conjugate at 1/2500 dilution
Predicted band size: 127 kDa
Observed band size: 145 kDa why is the actual band size different from the predicted?
Additional bands at: 80 kDa. We are unsure as to the identity of these extra bands. -
Anti-FHOD1 antibody (ab73443) at 1/500 dilution + K-562 cell lysate at 25 µg
Secondary
goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution
Predicted band size: 127 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted? -
Immunoperoxidase staining of formalin-fixed paraffin-embedded human spleen using ab73443, at 3 ug/ml of purified version of the antibody.
Protocols
Datasheets and documents
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Datasheet download
References (7)
ab73443 has been referenced in 7 publications.
- Jiang C et al. FHOD1 is upregulated in gastric cancer and promotes the proliferation and invasion of gastric cancer cells. Oncol Lett 22:712 (2021). PubMed: 34457067
- Zuidscherwoude M et al. Loss of mDia1 and Fhod1 impacts platelet formation but not platelet function. Platelets N/A:1-12 (2020). PubMed: 32981398
- Shi X et al. Active FHOD1 promotes the formation of functional actin stress fibers. Biochem J 476:2953-2963 (2019). PubMed: 31657439
- Schwartz C et al. Lamins and nesprin-1 mediate inside-out mechanical coupling in muscle cell precursors through FHOD1. Sci Rep 7:1253 (2017). WB . PubMed: 28455503
- Dwyer J et al. The formin FHOD1 in cardiomyocytes. Anat Rec (Hoboken) 297:1560-70 (2014). PubMed: 25125170
- Viswanatha R et al. Interactome analysis reveals ezrin can adopt multiple conformational states. J Biol Chem 288:35437-51 (2013). WB ; Human . PubMed: 24151071
- Ogembo JG et al. SIRPa/CD172a and FHOD1 are unique markers of littoral cells, a recently evolved major cell population of red pulp of human spleen. J Immunol 188:4496-505 (2012). IHC-P ; Human . PubMed: 22490440