Key features and details
- Rabbit polyclonal to FIBIN
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-FIBIN antibody
DescriptionRabbit polyclonal to FIBIN
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
- FIBIN over-expression HEK293T Lysate; Human placenta tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab122621 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Sequence similaritiesBelongs to the FIBIN family.
Cellular localizationSecreted. Golgi apparatus.
- Information by UniProt
- Fibin antibody
- FIBIN_HUMAN antibody
- Fin bud initiation factor homolog (zebrafish) antibody
Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm. Recommended concentration of ab122621 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab122621 at 1/50 dilution staining FIBIN in paraffin-embedded Human placenta tissue by Immunohistochemistry.
All lanes : Anti-FIBIN antibody (ab122621) at 1/250 dilution
Lane 1 : Negative control (vector only transfected HEK293T lysate)
Lane 2 : FIBIN Over-expression HEK293T Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)
Developed using the ECL technique.
ab122621 has not yet been referenced specifically in any publications.