Product nameAnti-Fibrillarin antibody [28F2]
See all Fibrillarin primary antibodies
DescriptionMouse monoclonal [28F2] to Fibrillarin
Tested applicationsSuitable for: WB, ICC/IF, IPmore details
Species reactivityReacts with: Saccharomyces cerevisiae
Nuclear preparation (S. cerevisiae). Hybridomas were screened by immunofluorescence on yeast cells and by western blotting on yeast protein homogenates.
General notesPfam number: PF01269
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.065% Sodium azide
Constituent: Tissue culture supernatant
PurityTissue culture supernatant
Purification notesSterile filtered.
Our Abpromise guarantee covers the use of ab4575 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use 2-4 µl for an amount of lysate derived from 5 OD600 units of yeast culture.
WB: 1/10000 (cell lysates)- 1/25000 (nuclear fractions)(yeast protein samples). Predicted molecular weight: 34 kDa. For other (non-ECL) western detection methods, 1/1000 - 1/5000.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionS-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (PubMed:24352239).
Sequence similaritiesBelongs to the methyltransferase superfamily. Fibrillarin family.
modificationsBy homology to other fibrillarins, some or all of the N-terminal domain arginines are modified to asymmetric dimethylarginine (DMA).
Cellular localizationNucleus, nucleolus. Fibrillar region of the nucleolus.
- Information by UniProt
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This product has been referenced in:
- Herzig B et al. Bällchen is required for self-renewal of germline stem cells in Drosophila melanogaster. Biol Open 3:510-21 (2014). Read more (PubMed: 24876388) »
- Merl J et al. Analysis of ribosome biogenesis factor-modules in yeast cells depleted from pre-ribosomes. Nucleic Acids Res 38:3068-80 (2010). ICC/IF . Read more (PubMed: 20100801) »