Product nameAnti-Fibrillarin antibody [4A4]
See all Fibrillarin primary antibodies
DescriptionMouse monoclonal [4A4] to Fibrillarin
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant full length protein corresponding to Human Fibrillarin aa 1-321. (Purified from E.coli).
MKPGFSPRGGGFGGRGGFGDRGGRGGRGGFGGGRGRGGGFRGRGRGGGGG GGGGGGGGRGGGGFHSGGNRGRGRGGKRGNQSGKNVMVEPHRHEGVFICR GKEDALVTKNLVPGESVYGEKRVSISEGDDKIEYRAWNPFRSKLAAAILG GVDQIHIKPGAKVLYLGAASGTTVSHVSDIVGPDGLVYAVEFSHRSGRDL INLAKKRTNIIPVIEDARHPHKYRMLIAMVDVIFADVAQPDQTRIVALNA HTFLRNGGHFVISIKANCIDSTASAEAVFASEVKKMQQENMKPQEQLTLE PYERDHAVVVGVYRPPPKVKN
Database link: P22087
- WB: Rat and mouse whole brain lysates; NIH/3T3, Hek293, HeLa and SH-SY5Y cell lysates. ICC/IF: HEK-293, C6 and HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.03% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab218846 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/5000. Predicted molecular weight: 33 kDa.|
|ICC/IF||1/1000 - 1/2000.|
FunctionS-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (PubMed:24352239).
Sequence similaritiesBelongs to the methyltransferase superfamily. Fibrillarin family.
modificationsBy homology to other fibrillarins, some or all of the N-terminal domain arginines are modified to asymmetric dimethylarginine (DMA).
Cellular localizationNucleus, nucleolus. Fibrillar region of the nucleolus.
- Information by UniProt
- 34 kD nucleolar scleroderma antigen antibody
- 34 kDa nucleolar scleroderma antigen antibody
- Fbl antibody
HEK-293 (human epithelial cell line from embryonic kidney transformed with large T antigen; Panel A) and C6 (rat glial tumor cell line; Panel B) cells stained for Fibrillarin (red) using ab218846 at 1/1000 dilution in ICC/IF. Co-stained with chicken pAb to vimentin, at 1/10000 dilution (green). The blue is DAPI staining of nuclear DNA.
All lanes : Anti-Fibrillarin antibody [4A4] (ab218846) at 1/2000 dilution
Lane 1 : Molecular weight standards
Lane 2 : Rat whole brain lysate
Lane 3 : Mouse whole brain lysate
Lane 4 : NIH/3T3 cell lysate
Lane 5 : Hek293 cell lysate
Lane 6 : HeLa cell lysate
Lane 7 : SH-SY5Y cell lysate
Predicted band size: 33 kDa
Fibrillarin detection is shown in green.
Also stained with a rabbit antibody to UCHL-1 at 1/3000 dilution (red).
Immunofluorescent analysis of HeLa cells labeling Fibrillarin with ab218846 at 1/1000 dilution (red). The cells were counterstained with a chicken antibody to vimentin at 1/5000 dilution (green) and DAPI to label the nuclei (blue).
ab218846 has not yet been referenced specifically in any publications.