Recombinant Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab166630)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10823(B)] to Fibrillarin - Nucleolar Marker
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker
See all Fibrillarin primary antibodies -
Description
Rabbit monoclonal [EPR10823(B)] to Fibrillarin - Nucleolar Marker -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human Fibrillarin aa 250 to the C-terminus.
Database link: P22087 -
Positive control
- 293T, HeLa, HepG2, HT-29 and MOLT4 cell lysates; ; HeLa cells. Permeabilized MOLT4 cells; Immunoprecipitation pellet from HepG2 whole cell lysate (ab7900). WB: 293T, HeLa, NIH/3T3 and PC-12 cell lysate; Mouse liver, spleen and testis tissue lysate. ICC/IF: NIH/3T3 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10823(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Alexa Fluor® 488 Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab184817)
- HRP Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab196980)
- Alexa Fluor® 568 Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab202540)
- Alexa Fluor® 594 Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab203400)
- Alexa Fluor® 555 Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab203410)
- Anti-Fibrillarin antibody [EPR10823(B)] - BSA and Azide free (ab240147)
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab166630 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB | (2) |
1/1000 - 1/5000. Predicted molecular weight: 33 kDa.
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ICC/IF |
1/5000.
For unpurified use at 1/100 - 1/250 |
Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/5000. Predicted molecular weight: 33 kDa. |
ICC/IF
1/5000. For unpurified use at 1/100 - 1/250 |
Target
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Function
S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (PubMed:24352239). -
Sequence similarities
Belongs to the methyltransferase superfamily. Fibrillarin family. -
Post-translational
modificationsBy homology to other fibrillarins, some or all of the N-terminal domain arginines are modified to asymmetric dimethylarginine (DMA). -
Cellular localization
Nucleus, nucleolus. Fibrillar region of the nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 2091 Human
- Entrez Gene: 102643269 Mouse
- Entrez Gene: 14113 Mouse
- Entrez Gene: 292747 Rat
- Omim: 134795 Human
- SwissProt: P22087 Human
- SwissProt: P35550 Mouse
- SwissProt: P22509 Rat
see all -
Alternative names
- 34 kD nucleolar scleroderma antigen antibody
- 34 kDa nucleolar scleroderma antigen antibody
- Fbl antibody
see all
Images
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling Fibrillarin with ab166630 at 1/200 (0.5 μg/ml) dilution followed by secondary antibody ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedused at 1/1000 dilution (2μg/ml) (Green). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 μg/ml) (Red). The Nuclear counterstain was DAPI (Blue).
Confocal image showing nuclear staining in NIH/3T3 cell line.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). -
All lanes : Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab166630) at 1/1000 dilution
Lane 1 : Mouse liver tissue lysate
Lane 2 : Mouse testis tissue lysate
Lane 3 : Mouse spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 33 kDa
Observed band size: 33 kDa
Exposure time: 81 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.
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All lanes : Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab166630) at 1/1000 dilution
Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : 293T (human embryonic kidney epithelial cell) whole cell lysate
Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 4 : PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 33 kDa
Observed band size: 33 kDa
Exposure time: 10 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.
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All lanes : Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab166630) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : MOLT4 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 33 kDa -
Immunocytochemistry/Immunofluorescence analysis of HT-29 (human colorectal adenocarcinoma) cells labelling Fibrillarin (green) with purified ab166630 at 1/5000. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as a nuclear counterstain.
Secondary Only Control: PBS was used instead of the primary antibody as the negative control.
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Immunofluorescent analysis of HeLa cells labeling Fibrillarin with ab166630 at a 1/100 dilution.
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Intracellular flow cytometric analysis of permeabilized MOLT4 cells using ab166630 at a 1/10 dilution (red) or a Rabbit IgG (negative) (green).
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Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab166630) at 1/1000 dilution + immunoprecipitation pellet from HepG2 cell lysate at 10 µg
Predicted band size: 33 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (18)
ab166630 has been referenced in 18 publications.
- Castells-Garcia A et al. Super resolution microscopy reveals how elongating RNA polymerase II and nascent RNA interact with nucleosome clutches. Nucleic Acids Res 50:175-190 (2022). PubMed: 34929735
- Luo W et al. Bioinformatic Analysis and In Vitro and In Vivo Experiments Reveal That Fibrillarin Participates in the Promotion of Lung Metastasis in Hepatocellular Carcinoma. Bioengineering (Basel) 9:N/A (2022). PubMed: 36004921
- Zhang J et al. Increased fibrillarin expression is associated with tumor progression and an unfavorable prognosis in hepatocellular carcinoma. Oncol Lett 21:92 (2021). PubMed: 33376525
- Weeks SE et al. Inhibiting ß-catenin disables nucleolar functions in triple-negative breast cancer. Cell Death Dis 12:242 (2021). PubMed: 33664239
- Yin X et al. RNA polymerase I subunit 12 plays opposite roles in cell proliferation and migration. Biochem Biophys Res Commun 560:112-118 (2021). PubMed: 33984768