Product nameAnti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (HRP)
See all Fibrillarin primary antibodies
DescriptionRabbit monoclonal [EPR10823(B)] to Fibrillarin - Nucleolar Marker (HRP)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to residues in Human Fibrillarin was used as an immunogen (UniProt ID: P22087). (The amino acid sequence is considered to be commercially sensitive).
- WB: HEK293, HeLa, HepG2 and MOLT4 whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.4
Preservative: 0.1% Proclin
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
- Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab166630)
- Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (Alexa Fluor® 488) (ab184817)
- Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (Alexa Fluor® 568) (ab202540)
- Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (Alexa Fluor® 594) (ab203400)
- Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (Alexa Fluor® 555) (ab203410)
- Anti-Fibrillarin antibody [EPR10823(B)] - BSA and Azide free (ab240147)
Our Abpromise guarantee covers the use of ab196980 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).|
FunctionS-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (PubMed:24352239).
Sequence similaritiesBelongs to the methyltransferase superfamily. Fibrillarin family.
modificationsBy homology to other fibrillarins, some or all of the N-terminal domain arginines are modified to asymmetric dimethylarginine (DMA).
Cellular localizationNucleus, nucleolus. Fibrillar region of the nucleolus.
- Information by UniProt
- 34 kD nucleolar scleroderma antigen antibody
- 34 kDa nucleolar scleroderma antigen antibody
- Fbl antibody
All lanes : Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (HRP) (ab196980) at 1/5000 dilution
Lane 1 : HEK293 (Human) Whole Cell Lysate (ab52256)
Lane 2 :
HeLa whole cell lysate (ab150035)
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 33 kDa
Exposure time: 8 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab196980 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab196980 has not yet been referenced specifically in any publications.