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This antibody is to fibrinogen alpha chain, but why then does it run so high in WB? The predicted MW of the alpha chain is 70 kDa and the alpha E chain is 95 kDa. What is the immunogen?
Asked on Feb 07 2012
Thank you for your inquiry.
I heard back from the supplying lab that the peptide maps approximately between amino acids 695 - 715 of human fibrinogen (alpha chain) (UniProt: P02671).
The predicted MW of the alpha chain is ˜91 kDa based on the fact that it is 831 a.a. long.
I hope this information helps. Please contact us with any other questions.
Answered on Feb 07 2012