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  1. Link

    fibrinogen-alpha-chain-antibody-epr2919-ab92572.pdf

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RecombinantRabMAb

Recombinant Anti-Fibrinogen alpha chain antibody [EPR2919] (ab92572)

  • Datasheet
  • SDS
Reviews (1)Q&A (2)References (7)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen alpha chain antibody [EPR2919] (ab92572)
  • Western blot - Anti-Fibrinogen alpha chain antibody [EPR2919] (ab92572)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen alpha chain antibody [EPR2919] (ab92572)
  • Immunocytochemistry/ Immunofluorescence - Anti-Fibrinogen alpha chain antibody [EPR2919] (ab92572)
  • Flow Cytometry (Intracellular) - Anti-Fibrinogen alpha chain antibody [EPR2919] (ab92572)
  • Western blot - Anti-Fibrinogen alpha chain antibody [EPR2919] (ab92572)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen alpha chain antibody [EPR2919] (ab92572)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen alpha chain antibody [EPR2919] (ab92572)
  • Immunoprecipitation - Anti-Fibrinogen alpha chain antibody [EPR2919] (ab92572)
  • Anti-Fibrinogen alpha chain antibody [EPR2919] (ab92572)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2919] to Fibrinogen alpha chain
  • Suitable for: WB, IP, IHC-P, ICC/IF, Flow Cyt (Intra)
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 594 APC Carrier Free HRP PE

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Overview

  • Product name

    Anti-Fibrinogen alpha chain antibody [EPR2919]
    See all Fibrinogen Alpha Chain primary antibodies
  • Description

    Rabbit monoclonal [EPR2919] to Fibrinogen alpha chain
  • Host species

    Rabbit
  • Specificity

    The immunogen is derived from isoform alpha-E, UniProt accession P02671-1, and the antibody is not expected to detect isoform alpha.
  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC/IF, Flow Cyt (Intra)more details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC: Mouse, rat and human placenta and mouse lung tissue; Flow Cyt (intra): Hepa1-6 cells; WB: Human, mouse and rat plasma lysates. Rat and mouse platelet lysates. Mouse liver tissue lysate; IP: Human plasma; ICC/IF: HepG2 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR2919
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Serum Proteins
    • Cardiovascular
    • Blood
    • Coagulation
    • Common
    • Immunology
    • Secreted Molecules
    • Other secreted molecules
    • Cardiovascular
    • Atherosclerosis
    • Thrombosis
    • Platelets
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Proteins
    • Other ECM Proteins

Associated products

  • Alternative Versions

    • Alexa Fluor® 594 Anti-Fibrinogen alpha chain antibody [EPR2919] (ab216367)
    • Anti-Fibrinogen alpha chain antibody [EPR2919] - BSA and Azide free (ab247586)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab92572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 95 kDa.
IP
1/50.
IHC-P (1)
1/3000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Antigen retrieval plus the use of an HRP/AP polymerized secondary antibody is highly recommended for enhanced staining.

For unupurified use at 1/50 - 1/1000

ICC/IF
1/50.

For unpurified use at 1/250.

Flow Cyt (Intra)
1/50.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 95 kDa.
IP
1/50.
IHC-P
1/3000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Antigen retrieval plus the use of an HRP/AP polymerized secondary antibody is highly recommended for enhanced staining.

For unupurified use at 1/50 - 1/1000

ICC/IF
1/50.

For unpurified use at 1/250.

Flow Cyt (Intra)
1/50.

Target

  • Function

    Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.
  • Tissue specificity

    Plasma.
  • Involvement in disease

    Defects in FGA are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This is a rare autosomal recessive disorder characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=The majority of cases of afibrinogenemia are due to truncating mutations. Variations in position Arg-35 (the site of cleavage of fibrinopeptide a by thrombin) leads to alpha-dysfibrinogenemias.
    Defects in FGA are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash.
  • Sequence similarities

    Contains 1 fibrinogen C-terminal domain.
  • Domain

    A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.
  • Post-translational
    modifications

    The alpha chain is not glycosylated.
    Forms F13A-mediated cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming fibronectin-fibrinogen heteropolymers.
    About one-third of the alpha chains in the molecules in blood were found to be phosphorylated.
    Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P02671 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2243 Human
    • Entrez Gene: 14161 Mouse
    • Entrez Gene: 361969 Rat
    • Omim: 134820 Human
    • SwissProt: P02671 Human
    • SwissProt: E9PV24 Mouse
    • SwissProt: P06399 Rat
    • Unigene: 351593 Human
    • Alternative names

      • Ac1873 antibody
      • Fba5e antibody
      • FGA antibody
      • Fib antibody
      • Fib2 antibody
      • FIBA_HUMAN antibody
      • Fibrinogen alpha chain antibody
      • Fibrinogen, A alpha polypeptide antibody
      • Fibrinogen--alpha polypeptide chain antibody
      • Fibrinopeptide A antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen alpha chain antibody [EPR2919] (ab92572)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen alpha chain antibody [EPR2919] (ab92572)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue sections labeling Fibrinogen alpha chain with purified ab92572 at 1/3000 dilution (0.053 µg/mL). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Western blot - Anti-Fibrinogen alpha chain antibody [EPR2919] (ab92572)
      Western blot - Anti-Fibrinogen alpha chain antibody [EPR2919] (ab92572)
      All lanes : Anti-Fibrinogen alpha chain antibody [EPR2919] (ab92572) at 1/10000 dilution (purified)

      Lane 1 : Human plasma lysate
      Lane 2 : Rat platelet lysate
      Lane 3 : Rat plasma lysate

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 95 kDa
      Observed band size: 95 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen alpha chain antibody [EPR2919] (ab92572)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen alpha chain antibody [EPR2919] (ab92572)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat placenta tissue sections labeling Fibrinogen alpha chain with purified ab92572 at 1/3000 dilution (0.053 µg/mL). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Immunocytochemistry/ Immunofluorescence - Anti-Fibrinogen alpha chain antibody [EPR2919] (ab92572)
      Immunocytochemistry/ Immunofluorescence - Anti-Fibrinogen alpha chain antibody [EPR2919] (ab92572)

      Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Fibrinogen alpha chain with purified ab92572 at 1/50 dilution (3.16 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% TritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    • Flow Cytometry (Intracellular) - Anti-Fibrinogen alpha chain antibody [EPR2919] (ab92572)
      Flow Cytometry (Intracellular) - Anti-Fibrinogen alpha chain antibody [EPR2919] (ab92572)

      Intracellular Intracellular Flow Cytometry analysis of Hepa1-6 (Mouse hepatoma epithelial cell) labeling Fibrinogen alpha chain with ab92572 at 1/50 (red). Goat anti rabbit IgG (Alexa Fluor® 488), ab150077 at 1/2000 was used as the secondary antibody. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control / colour Rabbit monoclonal IgG (ab172730) (black). Unlabelled control / colour Cell without incubation with primary antibody and secondary antibody (blue).

    • Western blot - Anti-Fibrinogen alpha chain antibody [EPR2919] (ab92572)
      Western blot - Anti-Fibrinogen alpha chain antibody [EPR2919] (ab92572)
      All lanes : Anti-Fibrinogen alpha chain antibody [EPR2919] (ab92572) at 1/1000 dilution

      Lane 1 : Mouse liver cell lysate
      Lane 2 : Mouse plasma cell lysate
      Lane 3 : Mouse platelet cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 95 kDa
      Observed band size: 87 kDa why is the actual band size different from the predicted?



      The band around 35 kD represents FGA fragment (PMID: 24937656).

      Exposure time:
      Lane 1: 3 minutes;
      Lanes 2-3: 5.5 seconds

       

      Blocking buffer and concentration: 5% NFDM/TBST
      Diluting buffer and concentration: 5% NFDM/TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen alpha chain antibody [EPR2919] (ab92572)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen alpha chain antibody [EPR2919] (ab92572)

      Immunohistochemical analysis of paraffin-embedded mouse placenta using ab92572 at 1/1000 dilution (0.5 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins. A rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as a secondary antibody (ready to use). Negative control used only the secondary antibody. Counterstained with haematoxylin.

      Positive staining in plasma of mouse placenta.

      The section was incubated with ab92572 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen alpha chain antibody [EPR2919] (ab92572)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen alpha chain antibody [EPR2919] (ab92572)

      Immunohistochemical analysis of paraffin-embedded mouse lung using ab92572 at 1/1000 dilution (0.5 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins, a rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as a secondary antibody (ready to use). Negative control used only the secondary antibody. Counterstained with haematoxylin.

      Strong positive staining in plasma of mouse lung (PMID: 26259584).

      The section was incubated with ab92572 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    • Immunoprecipitation - Anti-Fibrinogen alpha chain antibody [EPR2919] (ab92572)
      Immunoprecipitation - Anti-Fibrinogen alpha chain antibody [EPR2919] (ab92572)

      ab92572 at a dilution of 1/100 immunoprecipitating Fibrinogen alpha chain in human plasma.

      Lane 1 (input): Human plasma (10 µg)
      Lane 2 (+): ab92572 + human plasma.
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab92572 in human plasma.

      For western blotting, ab92572 was used as the primary antibody at a 1/1000 dilution and VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Anti-Fibrinogen alpha chain antibody [EPR2919] (ab92572)
      Anti-Fibrinogen alpha chain antibody [EPR2919] (ab92572)

    Protocols

    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (7)

    Publishing research using ab92572? Please let us know so that we can cite the reference in this datasheet.

    ab92572 has been referenced in 7 publications.

    • Allgoewer K  et al. New Proteomic Signatures to Distinguish Between Zika and Dengue Infections. Mol Cell Proteomics 20:100052 (2021). PubMed: 33582300
    • Calvo E  et al. Why Does COVID-19 Affect Patients with Spinal Cord Injury Milder? A Case-Control Study: Results from Two Observational Cohorts. J Pers Med 10:N/A (2020). PubMed: 33096722
    • Zheng X  et al. A circulating extracellular vesicles-based novel screening tool for colorectal cancer revealed by shotgun and data-independent acquisition mass spectrometry. J Extracell Vesicles 9:1750202 (2020). PubMed: 32363013
    • Uyama N  et al. Anti-interleukin-6 receptor antibody treatment ameliorates postoperative adhesion formation. Sci Rep 9:17558 (2019). PubMed: 31772282
    • Fetz AE  et al. Surface Area to Volume Ratio of Electrospun Polydioxanone Templates Regulates the Adsorption of Soluble Proteins from Human Serum. Bioengineering (Basel) 6:N/A (2019). PubMed: 31480458
    • Cheng P  et al. Prevascularization promotes endogenous cell-mediated angiogenesis by upregulating the expression of fibrinogen and connective tissue growth factor in tissue-engineered bone grafts. Stem Cell Res Ther 9:176 (2018). PubMed: 29973254
    • Rancourt RC  et al. Antifibrinolytic mechanisms in acute airway injury after sulfur mustard analog inhalation. Am J Respir Cell Mol Biol 51:559-67 (2014). PubMed: 24796565

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Fibrinogen alpha chain antibody [EPR2919]

    Poor
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Human tissue sections)
    Antigen retrieval step
    Heat mediated
    Permeabilization
    No
    Specification
    Human tissue sections
    Blocking step
    Normal Horse serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 24°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Jeffrey Browning

    Verified customer

    Submitted Dec 29 2020

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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