Recombinant Anti-Fibrinogen alpha chain antibody [EPR2919] (ab92572)

The band around 35 kD represents FGA fragment (PMID: 24937656).

Exposure time:
Lane 1: 3 minutes;
Lanes 2-3: 5.5 seconds

Blocking buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration: 5% NFDM/TBST

Immunohistochemical analysis of paraffin-embedded mouse placenta using ab92572 at 1/1000 dilution (0.5 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins. A rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as a secondary antibody (ready to use). Negative control used only the secondary antibody. Counterstained with haematoxylin.

Positive staining in plasma of mouse placenta.

The section was incubated with ab92572 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Immunocytochemistry/ Immunofluorescence analysis of  Hepa1-6 (mouse hepatoma epithelial cell) labeling Fibrinogen alpha chain with ab92572 at a dilution of 1/250. Confocal image showing cytoplasmic staining in Hepa1-6 cells. ab150077 AlexaFluor®488 Goat anti-Rabbit at 1/1000 dilution was used a secondary antibody. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% TritonX-100. ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at 1/200 was used as counterstain and nuclei were stained with DAPI (blue).

Immunohistochemical analysis of paraffin-embedded mouse lung using ab92572 at 1/1000 dilution (0.5 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins, a rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as a secondary antibody (ready to use). Negative control used only the secondary antibody. Counterstained with haematoxylin.

Strong positive staining in plasma of mouse lung (PMID: 26259584).

The section was incubated with ab92572 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Blocking and diluting buffer: 5% NFDM/TBST.

Immunocytochemistry/ Immunofluorescence analysis of HepG2 (human hepatocellular carcinoma epithelial cell) labeling Fibrinogen alpha chain with ab92572 at a dilution of 1/250. Confocal image showing cytoplasmic staining in HepG2 cells. ab150077 AlexaFluor^®488 Goat anti-Rabbit at 1/1000 dilution was used a secondary antibody. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% TritonX-100. ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at 1/200 was used as counterstain and nuclei were stained with DAPI (blue).

ab92572 at a dilution of 1/100 immunoprecipitating Fibrinogen alpha chain in human plasma.

Lane 1 (input): Human plasma (10 µg)
Lane 2 (+): ab92572 + human plasma.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab92572 in human plasma.

For western blotting, ab92572 was used as the primary antibody at a 1/1000 dilution and VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

Blocking and dilution buffer: 5% NFDM/TBST.