Key features and details
- Mouse monoclonal [UC45] to Fibrinogen alpha chain
- Suitable for: ICC, Flow Cyt, RIA, ELISA
- Reacts with: Mouse, Rat, Human
- Isotype: IgM
Product nameAnti-Fibrinogen alpha chain antibody [UC45]
See all Fibrinogen alpha chain primary antibodies
DescriptionMouse monoclonal [UC45] to Fibrinogen alpha chain
SpecificityThis antibody reacts very strongly with the alpha chain of human fibrinogen, for further information please see Hogg 1983 paper quoted.
Tested applicationsSuitable for: ICC, Flow Cyt, RIA, ELISAmore details
Species reactivityReacts with: Mouse, Rat, Human
Human acute monoblastic leukemia cells.
A monocyte and neuronal antigen present predominantly on a protein of 45kDa MW. UC45 is specific for monocytic leukemias. The relevant antigens are not expressed in all cases with monocytic differentiation.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from tissue culture supernatant.
Primary antibody notesA monocyte and neuronal antigen present predominantly on a protein of 45kDa MW. UC45 is specific for monocytic leukemias. The relevant antigens are not expressed in all cases with monocytic differentiation.
Our Abpromise guarantee covers the use of ab19079 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration.|
|Flow Cyt||Use 1µg for 106 cells.
ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
|RIA||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration. PubMed: 24959311|
FunctionFibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.
Involvement in diseaseDefects in FGA are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This is a rare autosomal recessive disorder characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=The majority of cases of afibrinogenemia are due to truncating mutations. Variations in position Arg-35 (the site of cleavage of fibrinopeptide a by thrombin) leads to alpha-dysfibrinogenemias.
Defects in FGA are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash.
Sequence similaritiesContains 1 fibrinogen C-terminal domain.
DomainA long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.
modificationsThe alpha chain is not glycosylated.
Forms F13A-mediated cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming fibronectin-fibrinogen heteropolymers.
About one-third of the alpha chains in the molecules in blood were found to be phosphorylated.
Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
Phosphorylation sites are present in the extracelllular medium.
- Information by UniProt
- Ac1873 antibody
- Fba5e antibody
- FGA antibody
Overlay histogram showing THP1 cells stained with ab19079 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab19079, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgM (mu chain) (ab97007) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
ab19079 has been referenced in 8 publications.
- Lv LL et al. Effect of haptoglobin on the treatment of chronic hepatitis B with interferon. Exp Ther Med 18:1417-1425 (2019). PubMed: 31316628
- Matsuoka A et al. Novel strategy of ovarian cancer implantation: Pre-invasive growth of fibrin-anchored cells with neovascularization. Cancer Sci 110:2658-2666 (2019). PubMed: 31199029
- Yeung AM et al. Fibrin glue inhibits migration of ocular surface epithelial cells. Eye (Lond) 30:1389-1394 (2016). PubMed: 27367746
- Richens JL et al. Practical detection of a definitive biomarker panel for Alzheimer's disease; comparisons between matched plasma and cerebrospinal fluid. Int J Mol Epidemiol Genet 5:53-70 (2014). ELISA ; Human . PubMed: 24959311
- Wu X et al. Molecular MRI of atherosclerotic plaque progression in an ApoE(-/-) mouse model with a CLT1 peptide targeted macrocyclic Gd(III) chelate. Am J Nucl Med Mol Imaging 3:446-55 (2013). PubMed: 24116353
- Linch DC et al. Monoclonal antibodies differentiating between monocytic and nonmonocytic variants of AML. Blood 63:566-73 (1984). PubMed: 6365201
- Hogg N Human monocytes are associated with the formation of fibrin. J Exp Med 157:473-85 (1983). PubMed: 6822786
- Hogg N et al. Monoclonal antibody with specificity for monocytes and neurons. Cell 24:875-84 (1981). PubMed: 6788378