Anti-Fibrinogen antibody (ab34269)

Rabbit polyclonal Fibrinogen antibody. Validated in ELISA, IHC, FuncS, ICC/IF, sELISA and tested in Mouse, Rabbit, Human. Cited in 16 publication(s). Independently reviewed in 3 review(s).

Overview

  • Product name

  • Description

    Rabbit polyclonal to Fibrinogen
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, ELISA, Functional Studies, Sandwich ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Rabbit, Human
  • Immunogen

    Purified fibrinogen from human plasma.

Properties

Applications

Our Abpromise guarantee covers the use of ab34269 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
ELISA 1/500.
Functional Studies Use at an assay dependent concentration. This antibody has been tested in plasma clotting assays. The plasma clotting time was prolonged (but not completely neutralized) by the antibody. Testing has indicated that these neutralisation assays work only when this antibody has been protein A column purified first.
Sandwich ELISA 1/200.

For sandwich ELISA, use this antibody as Detection at 1/200 dilution with Mouse monoclonal [1F7] to Fibrinopeptide A (ab14790) as Capture.

Target

  • Function

    Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.
  • Tissue specificity

    Plasma.
  • Involvement in disease

    Defects in FGA are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This is a rare autosomal recessive disorder characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=The majority of cases of afibrinogenemia are due to truncating mutations. Variations in position Arg-35 (the site of cleavage of fibrinopeptide a by thrombin) leads to alpha-dysfibrinogenemias.
    Defects in FGA are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash.
  • Sequence similarities

    Contains 1 fibrinogen C-terminal domain.
  • Domain

    A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.
  • Post-translational
    modifications

    The alpha chain is not glycosylated.
    Forms F13A-mediated cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming fibronectin-fibrinogen heteropolymers.
    About one-third of the alpha chains in the molecules in blood were found to be phosphorylated.
    Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
    Phosphorylation sites are present in the extracellular medium.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • FGA antibody
    • FGB antibody
    • FGG antibody
    • Fib2 antibody
    • FIBA_HUMAN antibody
    • Fibrinogen A alpha polypeptide antibody
    • Fibrinogen alpha chain antibody
    • Fibrinogen B alpha polypeptide antibody
    • Fibrinogen beta chain antibody
    • Fibrinogen G alpha polypeptide antibody
    • Fibrinogen gamma chain antibody
    • fibrinogen, B beta polypeptide antibody
    • fibrinogen, G gamma polypeptide antibody
    • fibrinogen, gamma polypeptide antibody
    • Fibrinogen--alpha -polypeptide chain antibody
    • Fibrinogen--beta -polypeptide chain antibody
    • Fibrinogen--gamma-polypeptide chain antibody
    see all

Images

  • Ab34269 staining human liver. Staining is localized to the cytoplasm.
    Left panel: with primary antibody at 1/50. Right panel: isotype control.
    Sections were stained using an automated system (Dako PT Link), at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer, citrate pH 6.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

  • Standard Curve for Fibrinogen (Analyte: Fibrinogen protein (Human) (ab84410)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [1F7] to Fibrinopeptide A (ab14790) at 5µg/ml and Detector Antibody Rabbit polyclonal to Fibrinogen (ab34269) at 1/2000.

  • ab34269 staining Fibrinogen in Human liver/placenta tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in formaldehyde and subjected to heat-mediated antigen retrieval in citrate buffer, pH 6.0 for 20 minutes at 100°C. The primary antibody was diluted 1/2000 and incubated with the sample for 20 minutes at 25°C. An HRP polymer-conjugated mouse anti-rabbit polyclonal IgG was used as the secondary antibody.

    See Abreview

  • Immunohistochemical analysis of rat heart, staining Fibrinogen with ab34269.

    Tissue was fixed with formaldehyde and blocked with 0.25% BSA for 15 minutes at room temperature; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1.5 µg/ml in 1% BSA in TBS) for 20 minutes. An undiluted HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:

See all 19 Publications for this product

Customer reviews and Q&As

1-10 of 11 Abreviews or Q&A

Application
IHC - Wholemount
Sample
Mouse Tissue (brain tissue)
Specification
brain tissue

Abcam user community

Verified customer

Submitted Sep 02 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cow Tissue sections (man-made and in vivo fibrin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: sodium citrate (10mM, pH 6.0)
Permeabilization
No
Specification
man-made and in vivo fibrin
Blocking step
DAKO Dual Endogenous enzyme block (15mins) followed by horse serum (1hr) as blocking agent for 1 hour(s) and 15 minute(s) · Concentration: 2.5% · Temperature: 23°C
Fixative
10% neutral-buffered formalin

Abcam user community

Verified customer

Submitted May 06 2019

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
Yes - 0.1% Triton-X
Specification
Brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Methanol:Acetone

Ms. Amy Gorman

Verified customer

Submitted Jul 15 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Heart/Plasma)
Specification
Heart/Plasma
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization
No
Blocking step
Protein Block (Casein) as blocking agent for 15 minute(s) · Concentration: 0.25% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Oct 18 2012

Question
Answer

DISCOUNT CODE: ***
Expiration date: February 12th, 2013
Value: $337

I am very pleased to hear you would like to accept our offer and test ab34269 in rat. This code will give you a discount off your next order before the expiration date. To redeem this offer, please submit an Abreview for rat and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/abtrial.

Read More

Answer

Thank you for contacting Abcam regarding ab34269.


This antibody is a whole antiserum derivedfrom rabbit, meaning, it has not been purified further. The antibody can be stored in this format in small aliquots at -20oC. The dilution buffer you use for your experiments will depend on the application you are using, but generally your blocking buffer or PBS is sufficient. I do not recommend adding goat serum to the antibody for any reason.


I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

Read More

Answer

Thank you for contacting us. The antibody ab34269 may be a good choice for what you describe, though it has not, to our knowledge been tested in exactly that way. The antibody is a whole serum, unpurified. The negative control for this, to account for binding of other serum elements, including immunoglobulins not specific for fibrinogen, would be serum from a non-immunized rabbit, such as ab7487:

Click here (or use the following: https://www.abcam.com/index.html?datasheet=7487).

You will need a secondary antibody, anti-rabbit IgG conjugated to a fluorochrome. For instance, for a green fluorochrome, we have ab96883:

Click here (or use the following: https://www.abcam.com/index.html?datasheet=96883).

Please let me know if you have any questions or concerns.

Read More

Question
Answer

Thank you for contacting Abcam.

I have talked to the lab and they have informed me that this antibody will recognize both Fibrinogen and Fibrin.

If there is anything else I can help you with, please let me know.

Read More

Answer

Thank you for your phone call. I am still waiting for information regarding the immunogens for ab92481 and ab92510, but I will let you know as soon as I have any additional information.

DISCOUNT CODE: ***

Expiration date: August 16th, 2012

I am very pleased to hear you would like to accept our offer and test ab34269 in ferret. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ferret and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (liver, placenta)
Specification
liver, placenta
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate, pH6, 20 min, 100C
Permeabilization
No

Abcam user community

Verified customer

Submitted Dec 02 2011

1-10 of 11 Abreviews or Q&A

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