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    fibrinogen-antibody-ab58207.pdf

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Cardiovascular Blood Platelets
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Anti-Fibrinogen antibody (ab58207)

  • Datasheet
Submit a review Q&A (2)References (6)

Product price, shipping and contact information

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen antibody (ab58207)
  • Western blot - Anti-Fibrinogen antibody (ab58207)

Key features and details

  • Mouse monoclonal to Fibrinogen
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

You may also be interested in

Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

View more associated products

Overview

  • Product name

    Anti-Fibrinogen antibody
    See all Fibrinogen primary antibodies
  • Description

    Mouse monoclonal to Fibrinogen
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment: RDNCCILDER FGSYCPTTCG IADFLSTYQT KVDKDLQSLE DILHQVENKT SEVKQLIKAI QLTYNPDESS KPNMIDAATL KSRKMLEEIM KYEASILTHD , corresponding to amino acids 31-130 of Human Fibrinogen Gamma

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    This product was changed from ascites to tissue culture supernatant on 12th of March 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.4
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Cardiovascular
    • Blood
    • Platelets
    • Cardiovascular
    • Blood
    • Coagulation
    • Common
    • Cardiovascular
    • Atherosclerosis
    • Thrombosis
    • Other
    • Metabolism
    • Types of disease
    • Metabolic disorders

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab58207 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
Application Abreviews Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 52 kDa.
IHC-P
Use at an assay dependent concentration.
Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 52 kDa.
IHC-P
Use at an assay dependent concentration.

Target

  • Function

    Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.
  • Tissue specificity

    Plasma.
  • Involvement in disease

    Defects in FGA are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This is a rare autosomal recessive disorder characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=The majority of cases of afibrinogenemia are due to truncating mutations. Variations in position Arg-35 (the site of cleavage of fibrinopeptide a by thrombin) leads to alpha-dysfibrinogenemias.
    Defects in FGA are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash.
  • Sequence similarities

    Contains 1 fibrinogen C-terminal domain.
  • Domain

    A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.
  • Post-translational
    modifications

    The alpha chain is not glycosylated.
    Forms F13A-mediated cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming fibronectin-fibrinogen heteropolymers.
    About one-third of the alpha chains in the molecules in blood were found to be phosphorylated.
    Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
    Phosphorylation sites are present in the extracellular medium.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P02671 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2243 Human
    • Entrez Gene: 2244 Human
    • Entrez Gene: 2266 Human
    • Omim: 134820 Human
    • Omim: 134830 Human
    • Omim: 134850 Human
    • SwissProt: P02671 Human
    • SwissProt: P02675 Human
    • SwissProt: P02679 Human
    • Unigene: 300774 Human
    • Unigene: 351593 Human
    • Unigene: 727584 Human
    see all
  • Alternative names

    • FGA antibody
    • FGB antibody
    • FGG antibody
    • Fib2 antibody
    • FIBA_HUMAN antibody
    • Fibrinogen A alpha polypeptide antibody
    • Fibrinogen alpha chain antibody
    • Fibrinogen B alpha polypeptide antibody
    • Fibrinogen beta chain antibody
    • Fibrinogen G alpha polypeptide antibody
    • Fibrinogen gamma chain antibody
    • fibrinogen, B beta polypeptide antibody
    • fibrinogen, G gamma polypeptide antibody
    • fibrinogen, gamma polypeptide antibody
    • Fibrinogen--alpha -polypeptide chain antibody
    • Fibrinogen--beta -polypeptide chain antibody
    • Fibrinogen--gamma-polypeptide chain antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen antibody (ab58207)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen antibody (ab58207)

    Fibrinogen antibody (ab58207) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human hepatocellular carcinoma tissue.

     

    This image was generated using the ascites version of the product.

  • Western blot - Anti-Fibrinogen antibody (ab58207)
    Western blot - Anti-Fibrinogen antibody (ab58207)

    Fibrinogen antibody (ab58207) at 1ug/lane + HeLa cell lysate at 25ug/lane.

     

    This image was generated using the ascites version of the product.

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (6)

    Publishing research using ab58207? Please let us know so that we can cite the reference in this datasheet.

    ab58207 has been referenced in 6 publications.

    • Niu J  et al. Aberrant oligodendroglial-vascular interactions disrupt the blood-brain barrier, triggering CNS inflammation. Nat Neurosci 22:709-718 (2019). PubMed: 30988524
    • Zhang Y  et al. Orphan nuclear receptor ERR? is a key regulator of human fibrinogen gene expression. PLoS One 12:e0182141 (2017). PubMed: 28750085
    • Petersen MA  et al. Fibrinogen Activates BMP Signaling in Oligodendrocyte Progenitor Cells and Inhibits Remyelination after Vascular Damage. Neuron 96:1003-1012.e7 (2017). Rat . PubMed: 29103804
    • Ohashi R  et al. Morphological spectrum of renal pathology and its correlation to clinical features in patients with disseminated intravascular coagulation: A study involving a series of 21 autopsy cases. Pathol Int 64:443-52 (2014). Human . PubMed: 25146453
    • Hametner S  et al. Iron and neurodegeneration in the multiple sclerosis brain. Ann Neurol 74:848-61 (2013). PubMed: 23868451
    • Carlson MA  et al. A totally recombinant human fibrin Sealant. J Surg Res N/A:N/A (2013). IHC-P ; Pig . PubMed: 24169144

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Question

    Regarding ab58207, does it require enzymatic pretreatment or heat pretreatment?

    Read More

    Abcam community

    Verified customer

    Asked on Feb 11 2013

    Answer


    We usually recommend a heat pretreatment using sodium citrate 10 mM, pH 6 or Tris/EDTA pH 9 for 5-10 minutes.

    Please visit https://www.abcam.com/index.html?pageconfig=resource&rid=11488 for more information on antigen retrieval.

    Read More

    Abcam Scientific Support

    Answered on Feb 11 2013

    Question

    Regarding ab58207 reactivity,
    Do you think it might be good for other species than Human?
    Do you think, based on immunogen sequence homology it might work for Mouse or Rat?

    Thank you for your kind support.
    Regards,

    Read More

    Abcam community

    Verified customer

    Asked on Jan 28 2013

    Answer

    Thank you for contacting us.

    The immunogen sequence homology with mouse or rat is only 71%-73% so it most likely that the antibody will not be cross reacting with these species. However if customer is interested in testing these species I can provide Abtrial discount code.

    http://www.ncbi.nlm.nih.gov/blast/Blast.cgi#alnHdr_19527078

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Jan 28 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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