Overview

  • Product name

    Anti-Fibrinogen beta chain antibody
    See all Fibrinogen beta chain primary antibodies
  • Description

    Rabbit polyclonal to Fibrinogen beta chain
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Pig
  • Immunogen

    Recombinant fragment corresponding to Human Fibrinogen beta chain aa 250-491.

  • Positive control

    • 293T, A431, H1299, HeLa, HepG2, MOLT4, Raji and Mouse liver whole cell lysates, HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab137830 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 56 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.
  • Involvement in disease

    Defects in FGB are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This rare autosomal recessive disorder is characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=Patients with congenital fibrinogen abnormalities can manifest different clinical pictures. Some cases are clinically silent, some show a tendency toward bleeding and some show a predisposition for thrombosis with or without bleeding.
  • Sequence similarities

    Contains 1 fibrinogen C-terminal domain.
  • Domain

    A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.
  • Post-translational
    modifications

    Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Beta fibrinogen antibody
    • Epididymis Secretory Sperm Binding Protein Li 78p antibody
    • FGB antibody
    • FIBB_HUMAN antibody
    • Fibrinogen beta chain antibody
    • Fibrinogen, B Beta Polypeptide antibody
    • HEL-S-78p antibody
    see all

Images

  • Anti-Fibrinogen beta chain antibody (ab137830) at 1/5000 dilution + Molt 4 whole cell lysate at 30 µg

    Predicted band size: 56 kDa



    7.5% SDS PAGE
  • Anti-Fibrinogen beta chain antibody (ab137830) at 1/5000 dilution + Mouse liver whole cell lysate at 50 µg

    Predicted band size: 56 kDa



    10% SDS PAGE
  • Immunofluorescence analysis of methanol-fixed HeLa cells, labelling Fibrinogen beta chain using ab137830 at 1/200 dilution. Bottom image is costained with Hoechst 33342.

References

ab137830 has not yet been referenced specifically in any publications.

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