Overview

  • Product name

    Anti-Fibrinogen beta chain antibody
    See all Fibrinogen beta chain primary antibodies
  • Description

    Goat polyclonal to Fibrinogen beta chain
  • Host species

    Goat
  • Specificity

    This antibody is expected to recognize both reported isoforms (NP_005132.2; NP_001171670.1).
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Fibrinogen beta chain aa 177-190 (internal sequence) (Cysteine residue). (NP_005132.2)
    Sequence:

    C-EKHQLYIDETVNSN


    Database link: P02675

  • Positive control

    • WB: Human liver tissue lysate. IHC-P: Human placenta and kidney tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.3
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab240442 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.01 - 0.03 µg/ml.
IHC-P Use a concentration of 5 µg/ml.

Steamed heat mediated antigen retrieval with citrate buffer, pH 6.0.

Target

  • Function

    Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.
  • Involvement in disease

    Defects in FGB are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This rare autosomal recessive disorder is characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=Patients with congenital fibrinogen abnormalities can manifest different clinical pictures. Some cases are clinically silent, some show a tendency toward bleeding and some show a predisposition for thrombosis with or without bleeding.
  • Sequence similarities

    Contains 1 fibrinogen C-terminal domain.
  • Domain

    A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.
  • Post-translational
    modifications

    Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Beta fibrinogen antibody
    • Epididymis Secretory Sperm Binding Protein Li 78p antibody
    • FGB antibody
    • FIBB_HUMAN antibody
    • Fibrinogen beta chain antibody
    • Fibrinogen, B Beta Polypeptide antibody
    • HEL-S-78p antibody
    see all

Images

  • Paraffin-embedded human kidney tissue stained for Fibrinogen beta chain using ab240442 at 5 μg/ml in immunohistochemical analysis. AP staining.

  • Paraffin-embedded human placenta tissue stained for Fibrinogen beta chain using ab240442 at 5 μg/ml in immunohistochemical analysis. AP staining.

  • Anti-Fibrinogen beta chain antibody (ab240442) at 0.01 µg/ml + Human liver tissue lysate (in RIPAA buffer) at 35 µg

    Developed using the ECL technique.

References

ab240442 has not yet been referenced specifically in any publications.

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