Recombinant Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18145-84] to Fibrinogen beta chain
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-Fibrinogen beta chain antibody [EPR18145-84]
See all Fibrinogen beta chain primary antibodies -
Description
Rabbit monoclonal [EPR18145-84] to Fibrinogen beta chain -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Human liver tissue lysate and plasma; rat liver, platelets and plasma; mouse liver and plasma; HepG2 and Hepa1-6 whole cell lysate. IHC-P: Mouse liver and spleen tissue; rat liver and spleen tissue.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18145-84 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab189490 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
|
|
IHC-P |
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This antibody is not recommended for human species in IHC application due to nonspecific staining on human stromal cells. |
Notes |
---|
WB
1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). |
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. This antibody is not recommended for human species in IHC application due to nonspecific staining on human stromal cells. |
Target
-
Function
Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation. -
Involvement in disease
Defects in FGB are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This rare autosomal recessive disorder is characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=Patients with congenital fibrinogen abnormalities can manifest different clinical pictures. Some cases are clinically silent, some show a tendency toward bleeding and some show a predisposition for thrombosis with or without bleeding. -
Sequence similarities
Contains 1 fibrinogen C-terminal domain. -
Domain
A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure. -
Post-translational
modificationsConversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 2244 Human
- Entrez Gene: 110135 Mouse
- Entrez Gene: 24366 Rat
- SwissProt: P02675 Human
- Unigene: 300774 Human
-
Alternative names
- Beta fibrinogen antibody
- Epididymis Secretory Sperm Binding Protein Li 78p antibody
- FGB antibody
see all
Images
-
All lanes : Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490) at 1/1000 dilution
Lane 1 : Human liver lysate
Lane 2 : Human plasma
Lane 3 : Rat plasma
Lane 4 : Rat platelet lysate
Lane 5 : Mouse plasma
Lane 6 : Rat liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lanes 1-2 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution
Lanes 3-6 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 55 kDa
Observed band size: 55 kDaBlocking and dilution buffer: 5% NFDM/TBST
Exposure times:
Lanes 1-4: 3 seconds
Lane 5: 2 seconds
Lane 6: 1 minute
-
All lanes : Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490) at 1/1000 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : Rat liver lysate
Lane 3 : Hepa1-6 (mouse hepatoma epithelial cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 55 kDa
Observed band size: 55 kDaBlocking and dilution buffer: 5% NFDM/TBST
Exposure times:
Lane 1: 3 seconds
Lane 2: 1 second
Lane 3: 3 minutes
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Fibrinogen beta with ab189490 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on hepatocytes and blood vessels of mouse liver (PMID: 10467729; PMID: 3778466) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Fibrinogen beta with ab189490 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on hepatocytes and blood vessels of mouse liver (PMID: 3778466) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)
Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Fibrinogen beta with ab189490 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on hepatocytes and blood vessels of mouse liver (PMID: 10467729; PMID: 3778466) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)
Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Fibrinogen beta with ab189490 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on hepatocytes and blood vessels of mouse liver (PMID: 3778466) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (5)
ab189490 has been referenced in 5 publications.
- Zhang C et al. Fibrinogen/AKT/Microfilament Axis Promotes Colitis by Enhancing Vascular Permeability. Cell Mol Gastroenterol Hepatol 11:683-696 (2021). PubMed: 33075564
- Venugopal J et al. Interleukin-1 receptor inhibition reduces stroke size in a murine model of sickle cell disease. Haematologica 106:2469-2477 (2021). PubMed: 32817286
- Passet B et al. The Prion-like protein Shadoo is involved in mouse embryonic and mammary development and differentiation. Sci Rep 10:6765 (2020). PubMed: 32317725
- Bochenek ML et al. Activated Endothelial TGFß1 Signaling Promotes Venous Thrombus Nonresolution in Mice Via Endothelin-1: Potential Role for Chronic Thromboembolic Pulmonary Hypertension. Circ Res 126:162-181 (2020). PubMed: 31747868
- Nanizawa E et al. Short-term high-fat diet intake leads to exacerbation of concanavalin A-induced liver injury through the induction of procoagulation state. Biochem Biophys Rep 22:100736 (2020). PubMed: 32083190