Recombinant Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)
![Western blot - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)](https://www.abcam.com/ps/products/189/ab189490/Images/ab189490-288797-anti-fibrinogen-antibody-epr18145-84-western-blot.jpg "Western blot - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18145-84] to Fibrinogen beta chain
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Fibrinogen beta chain antibody [EPR18145-84]
See all Fibrinogen beta chain primary antibodies -
Description
Rabbit monoclonal [EPR18145-84] to Fibrinogen beta chain -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human liver tissue lysate and plasma; rat liver, platelets and plasma; mouse liver and plasma; HepG2 and Hepa1-6 whole cell lysate. IHC-P: Mouse liver and spleen tissue; rat liver and spleen tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18145-84 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab189490 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
|
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| IHC-P |
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This antibody is not recommended for human species in IHC application due to nonspecific staining on human stromal cells. |
| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). |
|
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. This antibody is not recommended for human species in IHC application due to nonspecific staining on human stromal cells. |
Target
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Function
Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation. -
Involvement in disease
Defects in FGB are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This rare autosomal recessive disorder is characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=Patients with congenital fibrinogen abnormalities can manifest different clinical pictures. Some cases are clinically silent, some show a tendency toward bleeding and some show a predisposition for thrombosis with or without bleeding. -
Sequence similarities
Contains 1 fibrinogen C-terminal domain. -
Domain
A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure. -
Post-translational
modificationsConversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 2244 Human
- Entrez Gene: 110135 Mouse
- Entrez Gene: 24366 Rat
- SwissProt: P02675 Human
- Unigene: 300774 Human
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Alternative names
- Beta fibrinogen antibody
- Epididymis Secretory Sperm Binding Protein Li 78p antibody
- FGB antibody
see all
Images
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Western blot - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)All lanes : Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490) at 1/1000 dilution
Lane 1 : Human liver lysate
Lane 2 : Human plasma
Lane 3 : Rat plasma
Lane 4 : Rat platelet lysate
Lane 5 : Mouse plasma
Lane 6 : Rat liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lanes 1-2 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution
Lanes 3-6 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 55 kDa
Observed band size: 55 kDaBlocking and dilution buffer: 5% NFDM/TBST
Exposure times:
Lanes 1-4: 3 seconds
Lane 5: 2 seconds
Lane 6: 1 minute
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![Western blot - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)](https://www.abcam.com/ps/products/189/ab189490/Images/ab189490-288798-anti-fibrinogen-antibody-epr18145-84-western-blot.jpg)
Western blot - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)All lanes : Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490) at 1/1000 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : Rat liver lysate
Lane 3 : Hepa1-6 (mouse hepatoma epithelial cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 55 kDa
Observed band size: 55 kDaBlocking and dilution buffer: 5% NFDM/TBST
Exposure times:
Lane 1: 3 seconds
Lane 2: 1 second
Lane 3: 3 minutes
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)](https://www.abcam.com/ps/products/189/ab189490/Images/ab189490-288799-anti-fibrinogen-antibody-epr18145-84-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Fibrinogen beta with ab189490 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on hepatocytes and blood vessels of mouse liver (PMID: 10467729; PMID: 3778466) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)](https://www.abcam.com/ps/products/189/ab189490/Images/ab189490-288800-anti-fibrinogen-antibody-epr18145-84-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Fibrinogen beta with ab189490 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on hepatocytes and blood vessels of mouse liver (PMID: 3778466) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)](https://www.abcam.com/ps/products/189/ab189490/Images/ab189490-288801-anti-fibrinogen-antibody-epr18145-84-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Fibrinogen beta with ab189490 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on hepatocytes and blood vessels of mouse liver (PMID: 10467729; PMID: 3778466) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)](https://www.abcam.com/ps/products/189/ab189490/Images/ab189490-288802-anti-fibrinogen-antibody-epr18145-84-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Fibrinogen beta with ab189490 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on hepatocytes and blood vessels of mouse liver (PMID: 3778466) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)](https://www.abcam.com/ps/products/189/ab189490/Images/ab189490-7-benefits-of-recombinant-antibodies.png)
Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (5)
ab189490 has been referenced in 5 publications.
- Zhang C et al. Fibrinogen/AKT/Microfilament Axis Promotes Colitis by Enhancing Vascular Permeability. Cell Mol Gastroenterol Hepatol 11:683-696 (2021). PubMed: 33075564
- Venugopal J et al. Interleukin-1 receptor inhibition reduces stroke size in a murine model of sickle cell disease. Haematologica 106:2469-2477 (2021). PubMed: 32817286
- Passet B et al. The Prion-like protein Shadoo is involved in mouse embryonic and mammary development and differentiation. Sci Rep 10:6765 (2020). PubMed: 32317725
- Bochenek ML et al. Activated Endothelial TGFß1 Signaling Promotes Venous Thrombus Nonresolution in Mice Via Endothelin-1: Potential Role for Chronic Thromboembolic Pulmonary Hypertension. Circ Res 126:162-181 (2020). PubMed: 31747868
- Nanizawa E et al. Short-term high-fat diet intake leads to exacerbation of concanavalin A-induced liver injury through the induction of procoagulation state. Biochem Biophys Rep 22:100736 (2020). PubMed: 32083190