Recombinant Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)

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Western blot - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18145-84] to Fibrinogen beta chain
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat, Human

Carrier Free

Product name

Anti-Fibrinogen beta chain antibody [EPR18145-84]
See all Fibrinogen beta chain primary antibodies
Description

Rabbit monoclonal [EPR18145-84] to Fibrinogen beta chain
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-Pmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human liver tissue lysate and plasma; rat liver, platelets and plasma; mouse liver and plasma; HepG2 and Hepa1-6 whole cell lysate. IHC-P: Mouse liver and spleen tissue; rat liver and spleen tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18145-84
Isotype

IgG
Research areas

Alternative Versions
- Anti-Fibrinogen beta chain antibody [EPR18145-84] - BSA and Azide free (ab227063)
Compatible Secondaries
- VeriBlot for IP Detection Reagent (HRP) (ab131366)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Related Products
- Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab189490 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
IHC-P		1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. This antibody is not recommended for human species in IHC application due to nonspecific staining on human stromal cells.

Notes
WB 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
IHC-P 1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. This antibody is not recommended for human species in IHC application due to nonspecific staining on human stromal cells.

Function

Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.
Involvement in disease

Defects in FGB are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This rare autosomal recessive disorder is characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=Patients with congenital fibrinogen abnormalities can manifest different clinical pictures. Some cases are clinically silent, some show a tendency toward bleeding and some show a predisposition for thrombosis with or without bleeding.
Sequence similarities

Contains 1 fibrinogen C-terminal domain.
Domain

A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.
Post-translational
modifications

Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
Cellular localization

Secreted.
Target information above from: UniProt accession P02675 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 2244 Human
- Entrez Gene: 110135 Mouse
- Entrez Gene: 24366 Rat
- SwissProt: P02675 Human
- Unigene: 300774 Human
Alternative names
- Beta fibrinogen antibody
- Epididymis Secretory Sperm Binding Protein Li 78p antibody
- FGB antibody
- FIBB_HUMAN antibody
- Fibrinogen beta chain antibody
- Fibrinogen, B Beta Polypeptide antibody
- HEL-S-78p antibody
see all

Western blot - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)

All lanes : Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490) at 1/1000 dilution

Lane 1 : Human liver lysate
Lane 2 : Human plasma
Lane 3 : Rat plasma
Lane 4 : Rat platelet lysate
Lane 5 : Mouse plasma
Lane 6 : Rat liver lysate

Lysates/proteins at 20 µg per lane.

Secondary
Lanes 1-2 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution
Lanes 3-6 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 55 kDa
Observed band size: 55 kDa

Blocking and dilution buffer: 5% NFDM/TBST

Exposure times:

Lanes 1-4: 3 seconds

Lane 5: 2 seconds

Lane 6: 1 minute
Western blot - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)

All lanes : Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490) at 1/1000 dilution

Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : Rat liver lysate
Lane 3 : Hepa1-6 (mouse hepatoma epithelial cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 55 kDa
Observed band size: 55 kDa

Blocking and dilution buffer: 5% NFDM/TBST

Exposure times:

Lane 1: 3 seconds

Lane 2: 1 second

Lane 3: 3 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Fibrinogen beta with ab189490 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on hepatocytes and blood vessels of mouse liver (PMID: 10467729; PMID: 3778466) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Fibrinogen beta with ab189490 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on hepatocytes and blood vessels of mouse liver (PMID: 3778466) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Fibrinogen beta with ab189490 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on hepatocytes and blood vessels of mouse liver (PMID: 10467729; PMID: 3778466) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)

Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Fibrinogen beta with ab189490 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on hepatocytes and blood vessels of mouse liver (PMID: 3778466) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-Fibrinogen beta chain antibody [EPR18145-84] (ab189490)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheet download

Download

Publishing research using ab189490? Please let us know so that we can cite the reference in this datasheet.

ab189490 has been referenced in 5 publications.

Zhang C et al. Fibrinogen/AKT/Microfilament Axis Promotes Colitis by Enhancing Vascular Permeability. Cell Mol Gastroenterol Hepatol 11:683-696 (2021). PubMed: 33075564
Venugopal J et al. Interleukin-1 receptor inhibition reduces stroke size in a murine model of sickle cell disease. Haematologica 106:2469-2477 (2021). PubMed: 32817286
Passet B et al. The Prion-like protein Shadoo is involved in mouse embryonic and mammary development and differentiation. Sci Rep 10:6765 (2020). PubMed: 32317725
Bochenek ML et al. Activated Endothelial TGFß1 Signaling Promotes Venous Thrombus Nonresolution in Mice Via Endothelin-1: Potential Role for Chronic Thromboembolic Pulmonary Hypertension. Circ Res 126:162-181 (2020). PubMed: 31747868
Nanizawa E et al. Short-term high-fat diet intake leads to exacerbation of concanavalin A-induced liver injury through the induction of procoagulation state. Biochem Biophys Rep 22:100736 (2020). PubMed: 32083190

Customer reviews and Q&As

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Related Products

Applications

The Abpromise guarantee

Target

Function

Involvement in disease

Sequence similarities

Domain

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

Datasheet download

Certificate of Compliance

References (5)

Customer reviews and Q&As

Post-translational
modifications