Recombinant
RabMAb

Recombinant Anti-Fibrinogen beta chain antibody [EPR19765] (ab193932)

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Overview

  • Product name

    Anti-Fibrinogen beta chain antibody [EPR19765]
    See all Fibrinogen beta chain primary antibodies

  • Description

    Rabbit monoclonal [EPR19765] to Fibrinogen beta chain

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Full length native protein (purified) corresponding to Human Fibrinogen beta chain.
    Database link: P02675

  • Positive control

    • WB: HepG2 whole cell lysate; Human plasma and fetal liver tissue lysate. ICC/IF: HepG2 cells. IP: HepG2 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol, 59% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR19765

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab193932 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
ICC/IF 1/100.
IP 1/30.

Target

  • Function

    Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.

  • Involvement in disease

    Defects in FGB are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This rare autosomal recessive disorder is characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=Patients with congenital fibrinogen abnormalities can manifest different clinical pictures. Some cases are clinically silent, some show a tendency toward bleeding and some show a predisposition for thrombosis with or without bleeding.

  • Sequence similarities

    Contains 1 fibrinogen C-terminal domain.

  • Domain

    A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.

  • Post-translational
    modifications

    Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • Beta fibrinogen antibody
      • Epididymis Secretory Sperm Binding Protein Li 78p antibody
      • FGB antibody
      • FIBB_HUMAN antibody
      • Fibrinogen beta chain antibody
      • Fibrinogen, B Beta Polypeptide antibody
      • HEL-S-78p antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Fibrinogen beta chain antibody [EPR19765] (ab193932)
      Immunocytochemistry/ Immunofluorescence - Anti-Fibrinogen beta chain antibody [EPR19765] (ab193932)

      Immunofluorescent analysis of 4% PFA-fixed, 0.1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling Fibrinogen beta chain with ab193932  at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining of Fibrinogen beta on HepG2 cells.

      The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    • Western blot - Anti-Fibrinogen beta chain antibody [EPR19765] (ab193932)
      Western blot - Anti-Fibrinogen beta chain antibody [EPR19765] (ab193932)
      All lanes : Anti-Fibrinogen beta chain antibody [EPR19765] (ab193932) at 1/2000 dilution

      Lane 1 : His-tagged human fibrinogen alpha chain recombinant protein (aa401-866)
      Lane 2 : GST-His-tagged human fibrinogen beta chain recombinant protein (aa1-491)
      Lane 3 : GST-His-tagged human fibrinogen gamma chain recombinant protein (aa1-453)

      Lysates/proteins at 0.02 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Developed using the ECL technique.

      Predicted band size: 55 kDa
      Observed band size: 80 kDa
      why is the actual band size different from the predicted?


      Exposure time: 1 second


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-Fibrinogen beta chain antibody [EPR19765] (ab193932)
      Western blot - Anti-Fibrinogen beta chain antibody [EPR19765] (ab193932)
      All lanes : Anti-Fibrinogen beta chain antibody [EPR19765] (ab193932) at 1/1000 dilution

      Lane 1 : Human fetal liver tissue lysate
      Lane 2 : Human plasma
      Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Lanes 1-2 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
      Lane 3 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

      Developed using the ECL technique.

      Predicted band size: 55 kDa
      Observed band size: 55 kDa



      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure time: Lane 1: 5 seconds; Lane 2: 1 second; Lane 3: 3 minutes.

       

    • Immunoprecipitation - Anti-Fibrinogen beta chain antibody [EPR19765] (ab193932)
      Immunoprecipitation - Anti-Fibrinogen beta chain antibody [EPR19765] (ab193932)

      Fibrinogen beta chain was immunoprecipitated from 0.35 mg of HepG2 (human liver hepatocellular carcinoma cell line) lysate with ab193932 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab193932 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

      Lane 1: HepG2 whole cell lysate 10 µg (Input). 

      Lane 2: ab193932 IP in HepG2 whole cell lysate. 

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab193932 in HepG2 whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 30 seconds.

    References

    Publishing research using ab193932? Please let us know so that we can cite the reference in this datasheet.

    ab193932 has not yet been referenced specifically in any publications.

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