Recombinant
RabMAb

Recombinant Anti-Fibrinogen beta chain antibody [EPR3082] (ab92510)

Overview

  • Product name

    Anti-Fibrinogen beta chain antibody [EPR3082]
    See all Fibrinogen beta chain primary antibodies
  • Description

    Rabbit monoclonal [EPR3082] to Fibrinogen beta chain
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICCmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Fibrinogen beta chain aa 150-250. The exact sequence is proprietary.

  • Positive control

    • WB: Human prostate and Human plasma lysates IHC-P: Human liver tissue
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab92510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
ICC
  • Application notes
    ICC: 1/100.
    IHC-P: 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    WB: 1/5000 - 1/10000. Predicted molecular weight: 56 kDa.

    Is unsuitable for IP.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.
    • Involvement in disease

      Defects in FGB are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This rare autosomal recessive disorder is characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=Patients with congenital fibrinogen abnormalities can manifest different clinical pictures. Some cases are clinically silent, some show a tendency toward bleeding and some show a predisposition for thrombosis with or without bleeding.
    • Sequence similarities

      Contains 1 fibrinogen C-terminal domain.
    • Domain

      A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.
    • Post-translational
      modifications

      Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • Beta fibrinogen antibody
      • Epididymis Secretory Sperm Binding Protein Li 78p antibody
      • FGB antibody
      • FIBB_HUMAN antibody
      • Fibrinogen beta chain antibody
      • Fibrinogen, B Beta Polypeptide antibody
      • HEL-S-78p antibody
      see all

    Images

    • All lanes : Anti-Fibrinogen beta chain antibody [EPR3082] (ab92510) at 1/5000 dilution

      Lane 1 : Human prostate lysate
      Lane 2 : Human plasma lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 56 kDa

    • ab92510, at a 1/50 dilution, staining Fibrinogen beta chain in formalin fixed, paraffin embedded Human liver tissue by Immunohistochemistry. Detection: by DAB staining

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    References

    ab92510 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab92510.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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