Overview

  • Product name
    Anti-Fibrinogen beta chain antibody [EPR3083]
    See all Fibrinogen beta chain primary antibodies
  • Description
    Rabbit monoclonal [EPR3083] to Fibrinogen beta chain
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Fibrinogen beta chain aa 400-500 (C terminal).
    Database link: P02675

  • Positive control
    • Human plasma, Human prostrate and Rat platelet lysates; HeLa cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133359 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.
    • Involvement in disease
      Defects in FGB are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This rare autosomal recessive disorder is characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=Patients with congenital fibrinogen abnormalities can manifest different clinical pictures. Some cases are clinically silent, some show a tendency toward bleeding and some show a predisposition for thrombosis with or without bleeding.
    • Sequence similarities
      Contains 1 fibrinogen C-terminal domain.
    • Domain
      A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.
    • Post-translational
      modifications
      Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Beta fibrinogen antibody
      • Epididymis Secretory Sperm Binding Protein Li 78p antibody
      • FGB antibody
      • FIBB_HUMAN antibody
      • Fibrinogen beta chain antibody
      • Fibrinogen, B Beta Polypeptide antibody
      • HEL-S-78p antibody
      see all

    Images

    • All lanes : Anti-Fibrinogen beta chain antibody [EPR3083] (ab133359) at 1/1000 dilution

      Lane 1 : Human plasma lysate
      Lane 2 : Human prostate lysate
      Lane 3 : Rat platelet lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 56 kDa



      Actual band size : 56 kDa
    • Immunofluorescence analysis of Fibrinogen beta chain in HeLa cells, using ab133359 at a dilution of 1/100.

    References

    This product has been referenced in:
    • Fan SM  et al. Inducing hair follicle neogenesis with secreted proteins enriched in embryonic skin. Biomaterials 167:121-131 (2018). IHC-P ; Mouse . Read more (PubMed: 29567388) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab133359.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up