Recombinant
RabMAb

Recombinant Anti-Fibrinogen beta chain antibody [EPR3083] - BSA and Azide free (ab248494)

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Overview

  • Product name

    Anti-Fibrinogen beta chain antibody [EPR3083] - BSA and Azide free
    See all Fibrinogen beta chain primary antibodies

  • Description

    Rabbit monoclonal [EPR3083] to Fibrinogen beta chain - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, Flow Cytmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Fibrinogen beta chain aa 400-500 (C terminal). The exact sequence is proprietary.
    Database link: P02675

  • General notes

    Ab248494 is the carrier-free version of ab133359. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab248494 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Affinity purified

  • Clonality

    Monoclonal

  • Clone number

    EPR3083

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab248494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
Flow Cyt Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-P or IP.

    • Target

    • Function

      Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.

    • Involvement in disease

      Defects in FGB are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This rare autosomal recessive disorder is characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=Patients with congenital fibrinogen abnormalities can manifest different clinical pictures. Some cases are clinically silent, some show a tendency toward bleeding and some show a predisposition for thrombosis with or without bleeding.

    • Sequence similarities

      Contains 1 fibrinogen C-terminal domain.

    • Domain

      A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.

    • Post-translational
      modifications

      Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.

    • Cellular localization

      Secreted.
    • Information by UniProt

    • Database links

      • Alternative names

        • Beta fibrinogen antibody
        • Epididymis Secretory Sperm Binding Protein Li 78p antibody
        • FGB antibody
        • FIBB_HUMAN antibody
        • Fibrinogen beta chain antibody
        • Fibrinogen, B Beta Polypeptide antibody
        • HEL-S-78p antibody
        see all

      References

      ab248494 has not yet been referenced specifically in any publications.

      Publishing research using ab248494? Please let us know so that we can cite the reference in this datasheet.

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      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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