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RecombinantRabMAb

Recombinant Anti-Fibrinogen beta chain antibody [EPR3083] - BSA and Azide free (ab248494)

  • Datasheet
  • SDS
  • Certificate of Compliance
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Western blot - Anti-Fibrinogen beta chain antibody [EPR3083] - BSA and Azide free (ab248494)
  • Immunocytochemistry - Anti-Fibrinogen beta chain antibody [EPR3083] - BSA and Azide free (ab248494)
  • Anti-Fibrinogen beta chain antibody [EPR3083] - BSA and Azide free (ab248494)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3083] to Fibrinogen beta chain - BSA and Azide free
  • Suitable for: ICC, WB
  • Reacts with: Rat, Human

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Overview

  • Product name

    Anti-Fibrinogen beta chain antibody [EPR3083] - BSA and Azide free
    See all Fibrinogen beta chain primary antibodies
  • Description

    Rabbit monoclonal [EPR3083] to Fibrinogen beta chain - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab248494 is the carrier-free version of ab133359. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab248494 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR3083
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Coagulation
    • Common
    • Cardiovascular
    • Atherosclerosis
    • Thrombosis
    • Other

Associated products

  • Alternative Versions

    • Anti-Fibrinogen beta chain antibody [EPR3083] (ab133359)
    • Alexa Fluor® 488 Anti-Fibrinogen beta chain antibody [EPR3083] (ab208247)
    • HRP Anti-Fibrinogen beta chain antibody [EPR3083] (ab212218)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
  • Recombinant Protein

    • Recombinant Human Fibrinogen beta chain protein (ab202189)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab248494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
All applications
Mouse
Application Abreviews Notes
ICC
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
Notes
ICC
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.
    • Involvement in disease

      Defects in FGB are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This rare autosomal recessive disorder is characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=Patients with congenital fibrinogen abnormalities can manifest different clinical pictures. Some cases are clinically silent, some show a tendency toward bleeding and some show a predisposition for thrombosis with or without bleeding.
    • Sequence similarities

      Contains 1 fibrinogen C-terminal domain.
    • Domain

      A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.
    • Post-translational
      modifications

      Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P02675 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 2244 Human
      • Entrez Gene: 110135 Mouse
      • Entrez Gene: 24366 Rat
      • SwissProt: P02675 Human
      • Unigene: 300774 Human
      • Alternative names

        • Beta fibrinogen antibody
        • Epididymis Secretory Sperm Binding Protein Li 78p antibody
        • FGB antibody
        • FIBB_HUMAN antibody
        • Fibrinogen beta chain antibody
        • Fibrinogen, B Beta Polypeptide antibody
        • HEL-S-78p antibody
        see all

      Images

      • Western blot - Anti-Fibrinogen beta chain antibody [EPR3083] - BSA and Azide free (ab248494)
        Western blot - Anti-Fibrinogen beta chain antibody [EPR3083] - BSA and Azide free (ab248494)
        All lanes : Anti-Fibrinogen beta chain antibody [EPR3083] (ab133359) at 1/1000 dilution

        Lane 1 : Human plasma lysate
        Lane 3 : Rat platelet lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 56 kDa



        This data was developed using ab133359, the same antibody clone in a different buffer formulation.

        Actual band size: 56 kDa.

      • Immunocytochemistry - Anti-Fibrinogen beta chain antibody [EPR3083] - BSA and Azide free (ab248494)
        Immunocytochemistry - Anti-Fibrinogen beta chain antibody [EPR3083] - BSA and Azide free (ab248494)
        This data was developed using ab133359, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of Fibrinogen beta chain in HeLa cells, using ab133359 at a dilution of 1/100.
      • Anti-Fibrinogen beta chain antibody [EPR3083] - BSA and Azide free (ab248494)
        Anti-Fibrinogen beta chain antibody [EPR3083] - BSA and Azide free (ab248494)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      Certificate of Compliance

      To download a Certificate of Compliance, please enter your Lot number below:

      References (0)

      Publishing research using ab248494? Please let us know so that we can cite the reference in this datasheet.

      ab248494 has not yet been referenced specifically in any publications.

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