Recombinant
RabMAb

Recombinant Anti-Fibrinogen beta chain antibody [EPR3083] (HRP) (ab212218)

Overview

  • Product name

    Anti-Fibrinogen beta chain antibody [EPR3083] (HRP)
    See all Fibrinogen beta chain primary antibodies
  • Description

    Rabbit monoclonal [EPR3083] to Fibrinogen beta chain (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    within Human Fibrinogen beta chain aa 400 to the C-terminus. The exact sequence is proprietary.
    Database link: P02675

  • Positive control

    • WB: Human Platelet, Mouse Platelet, Human Plasma and Mouse Plasma lysates.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab212218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).

Target

  • Function

    Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.
  • Involvement in disease

    Defects in FGB are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This rare autosomal recessive disorder is characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=Patients with congenital fibrinogen abnormalities can manifest different clinical pictures. Some cases are clinically silent, some show a tendency toward bleeding and some show a predisposition for thrombosis with or without bleeding.
  • Sequence similarities

    Contains 1 fibrinogen C-terminal domain.
  • Domain

    A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.
  • Post-translational
    modifications

    Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Beta fibrinogen antibody
    • Epididymis Secretory Sperm Binding Protein Li 78p antibody
    • FGB antibody
    • FIBB_HUMAN antibody
    • Fibrinogen beta chain antibody
    • Fibrinogen, B Beta Polypeptide antibody
    • HEL-S-78p antibody
    see all

Images

  • All lanes : Anti-Fibrinogen beta chain antibody [EPR3083] (HRP) (ab212218) at 1/5000 dilution

    Lane 1 : Human Platelet lysate
    Lane 2 : Mouse Platelet lysate
    Lane 3 : Human Plasma lysate
    Lane 4 : Mouse Plasma lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 56 kDa
    Observed band size: 56 kDa


    Exposure time: 3 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab212218 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab212218 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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