Anti-Fibrinogen gamma chain antibody [5A6] (ab119948)

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Overview

  • Product name
    Anti-Fibrinogen gamma chain antibody [5A6]
    See all Fibrinogen gamma chain primary antibodies
  • Description
    Mouse monoclonal [5A6] to Fibrinogen gamma chain
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 210-437 of Human Fibrinogen gamma chain

  • Positive control
    • Recombinant Fibrinogen gamma chain protein, NIH3T3 cells
  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR240839-12 are from Tissue Culture Supernatant

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    Purified from tissue culture supernatant.
  • Clonality
    Monoclonal
  • Clone number
    5A6
  • Isotype
    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab119948 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 52 kDa.
ELISA 1/10000.
ICC/IF 1/200 - 1/1000.

Target

  • Function
    Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.
  • Involvement in disease
    Defects in FGG are a cause of thrombophilia.
    Defects in FGG are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. It is a rare autosomal recessive disorder characterized by complete absence of detectable fibrinogen.
  • Sequence similarities
    Contains 1 fibrinogen C-terminal domain.
  • Domain
    A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.
  • Post-translational
    modifications
    Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
    Sulfation of C-terminal tyrosines increases affinity for thrombin.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
    • Alternative names
      • FGG antibody
      • FIBG_HUMAN antibody
      • Fibrinogen gamma chain antibody
      • Fibrinogen gamma polypeptide antibody
      • fibrinogen gamma-b chain antibody
      see all

    Images

    • Western blot - Anti-Fibrinogen gamma chain antibody [5A6] (ab119948)
      Western blot - Anti-Fibrinogen gamma chain antibody [5A6] (ab119948)
      Anti-Fibrinogen gamma chain antibody [5A6] (ab119948) at 1/500 dilution + Recombinant Fibrinogen gamma chain protein

      Predicted band size: 52 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-Fibrinogen gamma chain antibody [5A6] (ab119948)
      Immunocytochemistry/ Immunofluorescence - Anti-Fibrinogen gamma chain antibody [5A6] (ab119948)
      Immunofluorescence analysis of NIH3T3 cells using ab119948 at a dilution of 1/200 (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
    • ELISA - Anti-Fibrinogen gamma chain antibody [5A6] (ab119948)
      ELISA - Anti-Fibrinogen gamma chain antibody [5A6] (ab119948)
      ELISA, using ab119948 at a dilution of 1/10000.

    References

    This product has been referenced in:
    • Wu L  et al. A proteomic analysis identifies candidate early biomarkers to predict ovarian hyperstimulation syndrome in polycystic ovarian syndrome patients. Mol Med Rep 16:272-280 (2017). Read more (PubMed: 28534980) »
    • Benito-Jardón M  et al. The fibronectin synergy site re-enforces cell adhesion and mediates a crosstalk between integrin classes. Elife 6:N/A (2017). Read more (PubMed: 28092265) »
    See all 4 Publications for this product

    Publishing research using ab119948? Please let us know so that we can cite the reference in this datasheet.

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    Western blot abreview for Anti-Fibrinogen gamma chain antibody [5A6]

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Liver)
    Loading amount
    20 µg
    Specification
    Liver
    Gel Running Conditions
    Reduced Non-Denaturing (Native) (4-20%)
    Blocking step
    Milk as blocking agent for 6 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More

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    Verified customer

    Submitted Dec 06 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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