Anti-Fibrinogen gamma chain antibody [5A6] (ab119948)

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Western blot - Anti-Fibrinogen gamma chain antibody [5A6] (ab119948)
  • Immunocytochemistry/ Immunofluorescence - Anti-Fibrinogen gamma chain antibody [5A6] (ab119948)
  • ELISA - Anti-Fibrinogen gamma chain antibody [5A6] (ab119948)

Key features and details

  • Mouse monoclonal [5A6] to Fibrinogen gamma chain
  • Suitable for: WB, ELISA, ICC/IF
  • Reacts with: Mouse, Human, Recombinant fragment
  • Isotype: IgG2b

Overview

  • Product name

    Anti-Fibrinogen gamma chain antibody [5A6]
    See all Fibrinogen gamma chain primary antibodies

  • Description

    Mouse monoclonal [5A6] to Fibrinogen gamma chain

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ELISA, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Human, Recombinant fragment

  • Immunogen

    Recombinant fragment, corresponding to amino acids 210-437 of Human Fibrinogen gamma chain

  • Positive control

    • Recombinant Fibrinogen gamma chain protein, NIH3T3 cells

  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR240839-12 are from Tissue Culture Supernatant

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.

  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    Purified from tissue culture supernatant.

  • Clonality

    Monoclonal

  • Clone number

    5A6

  • Isotype

    IgG2b

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab119948 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 52 kDa.
ELISA 1/10000.
ICC/IF 1/200 - 1/1000.

Target

  • Function

    Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.

  • Involvement in disease

    Defects in FGG are a cause of thrombophilia.
    Defects in FGG are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. It is a rare autosomal recessive disorder characterized by complete absence of detectable fibrinogen.

  • Sequence similarities

    Contains 1 fibrinogen C-terminal domain.

  • Domain

    A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.

  • Post-translational
    modifications

    Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
    Sulfation of C-terminal tyrosines increases affinity for thrombin.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • FGG antibody
      • FIBG_HUMAN antibody
      • Fibrinogen gamma chain antibody
      • Fibrinogen gamma polypeptide antibody
      • fibrinogen gamma-b chain antibody
      see all

    Images

    • Western blot - Anti-Fibrinogen gamma chain antibody [5A6] (ab119948)
      Western blot - Anti-Fibrinogen gamma chain antibody [5A6] (ab119948)
      Anti-Fibrinogen gamma chain antibody [5A6] (ab119948) at 1/500 dilution + Recombinant Fibrinogen gamma chain protein

      Predicted band size: 52 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-Fibrinogen gamma chain antibody [5A6] (ab119948)
      Immunocytochemistry/ Immunofluorescence - Anti-Fibrinogen gamma chain antibody [5A6] (ab119948)
      Immunofluorescence analysis of NIH3T3 cells using ab119948 at a dilution of 1/200 (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
    • ELISA - Anti-Fibrinogen gamma chain antibody [5A6] (ab119948)
      ELISA - Anti-Fibrinogen gamma chain antibody [5A6] (ab119948)
      ELISA, using ab119948 at a dilution of 1/10000.

    References (4)

    Publishing research using ab119948? Please let us know so that we can cite the reference in this datasheet.

    ab119948 has been referenced in 4 publications.

    • Wu L  et al. A proteomic analysis identifies candidate early biomarkers to predict ovarian hyperstimulation syndrome in polycystic ovarian syndrome patients. Mol Med Rep 16:272-280 (2017). PubMed: 28534980
    • Benito-Jardón M  et al. The fibronectin synergy site re-enforces cell adhesion and mediates a crosstalk between integrin classes. Elife 6:N/A (2017). PubMed: 28092265
    • Schunke KJ  et al. A novel atherothrombotic model of ischemic stroke induced by injection of collagen into the cerebral vasculature. J Neurosci Methods 239:65-74 (2015). PubMed: 25314906
    • Liu T  et al. Immobilization of heparin/poly-l-lysine nanoparticles on dopamine-coated surface to create a heparin density gradient for selective direction of platelet and vascular cells behavior. Acta Biomater 10:1940-54 (2014). PubMed: 24342042

    Customer reviews and Q&As

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    Western blot abreview for Anti-Fibrinogen gamma chain antibody [5A6]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Liver)
    Loading amount
    20 µg
    Specification
    Liver
    Gel Running Conditions
    Reduced Non-Denaturing (Native) (4-20%)
    Blocking step
    Milk as blocking agent for 6 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More

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    Verified customer

    Submitted Dec 06 2012

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