Key features and details
- Mouse monoclonal [5A6] to Fibrinogen gamma chain
- Suitable for: WB, ELISA, ICC/IF
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG2b
Product nameAnti-Fibrinogen gamma chain antibody [5A6]
See all Fibrinogen gamma chain primary antibodies
DescriptionMouse monoclonal [5A6] to Fibrinogen gamma chain
Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
Species reactivityReacts with: Mouse, Human, Recombinant fragment
Recombinant fragment, corresponding to amino acids 210-437 of Human Fibrinogen gamma chain
- Recombinant Fibrinogen gamma chain protein, NIH3T3 cells
This product was changed from ascites to supernatant. Lot no’s high than GR240839-12 are from Tissue Culture Supernatant
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
Storage bufferPreservative: 0.05% Sodium azide
Concentration information loading...
PurityProtein G purified
Purification notesPurified from tissue culture supernatant.
Our Abpromise guarantee covers the use of ab119948 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 52 kDa.|
|ICC/IF||1/200 - 1/1000.|
FunctionFibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.
Involvement in diseaseDefects in FGG are a cause of thrombophilia.
Defects in FGG are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. It is a rare autosomal recessive disorder characterized by complete absence of detectable fibrinogen.
Sequence similaritiesContains 1 fibrinogen C-terminal domain.
DomainA long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.
modificationsConversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
Sulfation of C-terminal tyrosines increases affinity for thrombin.
- Information by UniProt
- FGG antibody
- FIBG_HUMAN antibody
- Fibrinogen gamma chain antibody
Anti-Fibrinogen gamma chain antibody [5A6] (ab119948) at 1/500 dilution + Recombinant Fibrinogen gamma chain protein
Predicted band size: 52 kDa
Immunofluorescence analysis of NIH3T3 cells using ab119948 at a dilution of 1/200 (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
ELISA, using ab119948 at a dilution of 1/10000.
ab119948 has been referenced in 4 publications.
- Wu L et al. A proteomic analysis identifies candidate early biomarkers to predict ovarian hyperstimulation syndrome in polycystic ovarian syndrome patients. Mol Med Rep 16:272-280 (2017). PubMed: 28534980
- Benito-Jardón M et al. The fibronectin synergy site re-enforces cell adhesion and mediates a crosstalk between integrin classes. Elife 6:N/A (2017). PubMed: 28092265
- Schunke KJ et al. A novel atherothrombotic model of ischemic stroke induced by injection of collagen into the cerebral vasculature. J Neurosci Methods 239:65-74 (2015). PubMed: 25314906
- Liu T et al. Immobilization of heparin/poly-l-lysine nanoparticles on dopamine-coated surface to create a heparin density gradient for selective direction of platelet and vascular cells behavior. Acta Biomater 10:1940-54 (2014). PubMed: 24342042