Western blot abreview for Anti-Fibrinogen gamma chain antibody [5A6]

Excellent
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Loading amount
20 µg
Specification
Liver
Gel Running Conditions
Reduced Non-Denaturing (Native) (4-20%)
Blocking step
Milk as blocking agent for 6 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Other product details

Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 24°C

Secondary antibody

Name
Non-Abcam antibody was used: Goat Anti-Mouse IgG (whole molecule) Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL+
Exposure
30 second(s)
Bands
Specific: 55 kDa Non-specific: 25 kDa

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Submitted Dec 06 2012

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