Key features and details
- Rabbit polyclonal to Fibrinogen gamma chain
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Fibrinogen gamma chain antibody
See all Fibrinogen gamma chain primary antibodies
DescriptionRabbit polyclonal to Fibrinogen gamma chain
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Pig
Recombinant fragment, corresponding to a region within amino acids 194-368 of Human Fibrinogen gamma chain.
- 293T, A431, H1299, HeLa, HepG2, Molt4, Raji cell lysates; HeLa cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab137728 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/300. Predicted molecular weight: 52 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionFibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.
Involvement in diseaseDefects in FGG are a cause of thrombophilia.
Defects in FGG are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. It is a rare autosomal recessive disorder characterized by complete absence of detectable fibrinogen.
Sequence similaritiesContains 1 fibrinogen C-terminal domain.
DomainA long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.
modificationsConversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
Sulfation of C-terminal tyrosines increases affinity for thrombin.
- Information by UniProt
- FGG antibody
- FIBG_HUMAN antibody
- Fibrinogen gamma chain antibody
All lanes : Anti-Fibrinogen gamma chain antibody (ab137728) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 52 kDa
10% SDS PAGE
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labelling Fibrinogen gamma chain with ab137728 at 1/200 dilution. Lower image is co-stained with Hoechst 33342.
ab137728 has not yet been referenced specifically in any publications.