Anti-Fibrinogen gamma chain antibody (ab137728)
Key features and details
- Rabbit polyclonal to Fibrinogen gamma chain
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Fibrinogen gamma chain antibody
See all Fibrinogen gamma chain primary antibodies -
Description
Rabbit polyclonal to Fibrinogen gamma chain -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Pig -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 194-368 of Human Fibrinogen gamma chain.
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Positive control
- 293T, A431, H1299, HeLa, HepG2, Molt4, Raji cell lysates; HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137728 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/300. Predicted molecular weight: 52 kDa.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/300. Predicted molecular weight: 52 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation. -
Involvement in disease
Defects in FGG are a cause of thrombophilia.
Defects in FGG are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. It is a rare autosomal recessive disorder characterized by complete absence of detectable fibrinogen. -
Sequence similarities
Contains 1 fibrinogen C-terminal domain. -
Domain
A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure. -
Post-translational
modificationsConversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
Sulfation of C-terminal tyrosines increases affinity for thrombin. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 2266 Human
- Entrez Gene: 99571 Mouse
- Entrez Gene: 403164 Pig
- Entrez Gene: 24367 Rat
- Omim: 134850 Human
- SwissProt: P02679 Human
- SwissProt: Q8VCM7 Mouse
- SwissProt: P02680 Rat
see all -
Alternative names
- FGG antibody
- FIBG_HUMAN antibody
- Fibrinogen gamma chain antibody
see all
Images
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All lanes : Anti-Fibrinogen gamma chain antibody (ab137728) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 52 kDa
10% SDS PAGE -
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labelling Fibrinogen gamma chain with ab137728 at 1/200 dilution. Lower image is co-stained with Hoechst 33342.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab137728 has been referenced in 1 publication.
- Buitrago L et al. Dominant role of aIIbß3 in platelet interactions with cross-linked fibrin fragment D-dimer. Blood Adv 4:2939-2949 (2020). PubMed: 32603423