Overview

  • Product name

    Anti-Fibrinogen gamma chain antibody
    See all Fibrinogen gamma chain primary antibodies
  • Description

    Rabbit polyclonal to Fibrinogen gamma chain
  • Host species

    Rabbit
  • Specificity

    ab62527 will bind to the fibrin gamma chain as well as the fibrinogen gamma chain.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 289-338 of Human Fibrinogen gamma chain (AMFKVGPEAD KYRLTYAYFA GGDAGDAFDG FDFGDDPSDK FFTSHNGMQF)

  • Positive control

    • Fetal liver cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab62527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function

    Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.
  • Involvement in disease

    Defects in FGG are a cause of thrombophilia.
    Defects in FGG are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. It is a rare autosomal recessive disorder characterized by complete absence of detectable fibrinogen.
  • Sequence similarities

    Contains 1 fibrinogen C-terminal domain.
  • Domain

    A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.
  • Post-translational
    modifications

    Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
    Sulfation of C-terminal tyrosines increases affinity for thrombin.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • FGG antibody
    • FIBG_HUMAN antibody
    • Fibrinogen gamma chain antibody
    • Fibrinogen gamma polypeptide antibody
    • fibrinogen gamma-b chain antibody
    see all

Images

  • Anti-Fibrinogen gamma chain antibody (ab62527) at 2.5 µg/ml + Fetal liver cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 52 kDa
    Observed band size: 52 kDa



    Gel concentration: 12%

References

This product has been referenced in:

  • Du C  et al. Isobaric tags for relative and absolute quantitation-based proteomics reveals potential novel biomarkers for the early diagnosis of acute myocardial infarction within 3 h. Int J Mol Med 43:1991-2004 (2019). Read more (PubMed: 30896787) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - whole (Spleen)
Loading amount
10 µg
Specification
Spleen
Treatment
FasL for 5 hours
Gel Running Conditions
Reduced Non-Denaturing (Native) (4-20%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C

Abcam user community

Verified customer

Submitted May 23 2012

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