Anti-Fibroblast activation protein, alpha antibody (ab137549)

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Western blot - Anti-Fibroblast activation protein, alpha antibody (ab137549)

    Key features and details

    • Rabbit polyclonal to Fibroblast activation protein, alpha
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-Fibroblast activation protein, alpha antibody
      See all Fibroblast activation protein, alpha primary antibodies

    • Description

      Rabbit polyclonal to Fibroblast activation protein, alpha

    • Host species

      Rabbit

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat, Cow

    • Immunogen

      Recombinant fragment, corresponding to a region within amino acids 95 - 377 of Human Fibroblast activation protein, alpha (Q12884).

    • Positive control

      • HepG2 cell lysate; Molt-4 cell lysate; Raji, 293T and A431 cell lines.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab137549 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/500 - 1/3000. Predicted molecular weight: 88 kDa.

    Target

    • Function

      In association with DPP4 is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers.

    • Tissue specificity

      Fibroblast specific.

    • Sequence similarities

      Belongs to the peptidase S9B family.

    • Post-translational
      modifications

      N-glycosylated.
      The N-terminus may be blocked.

    • Cellular localization

      Cell membrane. Cell projection > lamellipodium membrane. Cell projection > invadopodium membrane. Found in cell surface lamellipodia, invadopodia and on shed vesicles. Colocalized with DPP4 in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with DPP4 on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • 170 kDa melanoma membrane bound gelatinase antibody
      • 170 kDa melanoma membrane-bound gelatinase antibody
      • DPPIV antibody
      • FAP antibody
      • FAPA antibody
      • Fibroblast activation protein alpha antibody
      • Integral membrane serine protease antibody
      • SEPR_HUMAN antibody
      • Seprase antibody
      see all

    Images

    • Western blot - Anti-Fibroblast activation protein, alpha antibody (ab137549)
      Western blot - Anti-Fibroblast activation protein, alpha antibody (ab137549)
      All lanes : Anti-Fibroblast activation protein, alpha antibody (ab137549) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : Molt-4 cell lysate

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 88 kDa

    References (0)

    Publishing research using ab137549? Please let us know so that we can cite the reference in this datasheet.

    ab137549 has not yet been referenced specifically in any publications.

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