Anti-Fibroblast activation protein, alpha antibody (ab218164)
Key features and details
- Rabbit polyclonal to Fibroblast activation protein, alpha
- Suitable for: IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Fibroblast activation protein, alpha antibody
See all Fibroblast activation protein, alpha primary antibodies -
Description
Rabbit polyclonal to Fibroblast activation protein, alpha -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide within Human Fibroblast activation protein, alpha aa 340-390 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q12884 -
Positive control
- Mouse intestine and kidney tissues; human gastric cancer tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab218164 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. When using a fluorescent probe the recommended dilution is 1/50 – 1/200. |
Target
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Function
In association with DPP4 is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers. -
Tissue specificity
Fibroblast specific. -
Sequence similarities
Belongs to the peptidase S9B family. -
Post-translational
modificationsN-glycosylated.
The N-terminus may be blocked. -
Cellular localization
Cell membrane. Cell projection > lamellipodium membrane. Cell projection > invadopodium membrane. Found in cell surface lamellipodia, invadopodia and on shed vesicles. Colocalized with DPP4 in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with DPP4 on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma. - Information by UniProt
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Database links
- Entrez Gene: 2191 Human
- Entrez Gene: 14089 Mouse
- Entrez Gene: 192203 Rat
- Omim: 600403 Human
- SwissProt: Q12884 Human
- SwissProt: P97321 Mouse
- Unigene: 654370 Human
- Unigene: 41816 Mouse
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Alternative names
- 170 kDa melanoma membrane bound gelatinase antibody
- 170 kDa melanoma membrane-bound gelatinase antibody
- DPPIV antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody (ab218164)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse intestine tissue labeling Fibroblast activation protein, alpha with ab218164 at 1/200 dilution overnight at 4°C, followed by Goat Anti-Rabbit IgG, Cy3 conjugated at 1/200 dilution for 40 minutes at 37°C. DAPI was used to stain the cell nuclei.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody (ab218164)Immunohistochemical analysis of paraformaldehyde-fixed, paraffin-embedded mouse kidney tissue labeling Fibroblast activation protein, alpha with ab218164 at 1/200 dilution overnight at 4°C, followed by a conjugated secondary and DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody (ab218164)Immunohistochemical analysis of Paraformaldehyde-fixed, paraffin-embedded human gastric cancer tissue labeling Fibroblast activation protein, alpha with ab218164 at 1/200 dilution overnight at 4°C, followed by a conjugated secondary and DAB staining.
Datasheets and documents
References (0)
ab218164 has not yet been referenced specifically in any publications.
