Key features and details
- Rabbit polyclonal to Fibroblast activation protein, alpha
- Suitable for: IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Fibroblast activation protein, alpha antibody
See all Fibroblast activation protein, alpha primary antibodies
DescriptionRabbit polyclonal to Fibroblast activation protein, alpha
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Synthetic peptide within Human Fibroblast activation protein, alpha aa 340-390 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q12884
- Mouse intestine and kidney tissues; human gastric cancer tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab218164 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
When using a fluorescent probe the recommended dilution is 1/50 – 1/200.
FunctionIn association with DPP4 is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers.
Tissue specificityFibroblast specific.
Sequence similaritiesBelongs to the peptidase S9B family.
The N-terminus may be blocked.
Cellular localizationCell membrane. Cell projection > lamellipodium membrane. Cell projection > invadopodium membrane. Found in cell surface lamellipodia, invadopodia and on shed vesicles. Colocalized with DPP4 in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with DPP4 on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma.
- Information by UniProt
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse intestine tissue labeling Fibroblast activation protein, alpha with ab218164 at 1/200 dilution overnight at 4°C, followed by Goat Anti-Rabbit IgG, Cy3 conjugated at 1/200 dilution for 40 minutes at 37°C. DAPI was used to stain the cell nuclei.
Immunohistochemical analysis of paraformaldehyde-fixed, paraffin-embedded mouse kidney tissue labeling Fibroblast activation protein, alpha with ab218164 at 1/200 dilution overnight at 4°C, followed by a conjugated secondary and DAB staining.
Immunohistochemical analysis of Paraformaldehyde-fixed, paraffin-embedded human gastric cancer tissue labeling Fibroblast activation protein, alpha with ab218164 at 1/200 dilution overnight at 4°C, followed by a conjugated secondary and DAB staining.
ab218164 has not yet been referenced specifically in any publications.