Anti-Fibroblast activation protein, alpha antibody (ab218164)

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Overview

  • Product name

    Anti-Fibroblast activation protein, alpha antibody
    See all Fibroblast activation protein, alpha primary antibodies

  • Description

    Rabbit polyclonal to Fibroblast activation protein, alpha

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat

  • Immunogen

    Synthetic peptide within Human Fibroblast activation protein, alpha aa 340-390 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: Q12884

  • Positive control

    • Mouse intestine and kidney tissues; human gastric cancer tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab218164 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

When using a fluorescent probe the recommended dilution is 1/50 – 1/200.

Target

  • Function

    In association with DPP4 is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers.

  • Tissue specificity

    Fibroblast specific.

  • Sequence similarities

    Belongs to the peptidase S9B family.

  • Post-translational
    modifications

    N-glycosylated.
    The N-terminus may be blocked.

  • Cellular localization

    Cell membrane. Cell projection > lamellipodium membrane. Cell projection > invadopodium membrane. Found in cell surface lamellipodia, invadopodia and on shed vesicles. Colocalized with DPP4 in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with DPP4 on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma.
  • Information by UniProt

  • Database links

    • Alternative names

      • 170 kDa melanoma membrane bound gelatinase antibody
      • 170 kDa melanoma membrane-bound gelatinase antibody
      • DPPIV antibody
      • FAP antibody
      • FAPA antibody
      • Fibroblast activation protein alpha antibody
      • Integral membrane serine protease antibody
      • SEPR_HUMAN antibody
      • Seprase antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody (ab218164)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody (ab218164)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse intestine tissue labeling Fibroblast activation protein, alpha with ab218164 at 1/200 dilution overnight at 4°C, followed by Goat Anti-Rabbit IgG, Cy3 conjugated at 1/200 dilution for 40 minutes at 37°C. DAPI was used to stain the cell nuclei.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody (ab218164)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody (ab218164)

      Immunohistochemical analysis of paraformaldehyde-fixed, paraffin-embedded mouse kidney tissue labeling Fibroblast activation protein, alpha with ab218164 at 1/200 dilution overnight at 4°C, followed by a conjugated secondary and DAB staining.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody (ab218164)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody (ab218164)

      Immunohistochemical analysis of Paraformaldehyde-fixed, paraffin-embedded human gastric cancer tissue labeling Fibroblast activation protein, alpha with ab218164 at 1/200 dilution overnight at 4°C, followed by a conjugated secondary and DAB staining.

    References

    ab218164 has not yet been referenced specifically in any publications.

    Publishing research using ab218164? Please let us know so that we can cite the reference in this datasheet.

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