Anti-Fibroblast activation protein, alpha antibody (ab28244)


  • Product name
    Anti-Fibroblast activation protein, alpha antibody
    See all Fibroblast activation protein, alpha primary antibodies
  • Description
    Rabbit polyclonal to Fibroblast activation protein, alpha
  • Host species
  • Specificity
    This antibody recognizes the Stalk Region of both long isoforms of Fibroblast activation protein, alpha but does not recognize other DPP family members.
  • Tested applications
    Suitable for: ICC/IF, IHC-P, Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Fibroblast activation protein, alpha. The exact sequence is proprietary.



Our Abpromise guarantee covers the use of ab28244 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 10 µg/ml.
IHC-P Use at an assay dependent concentration. PubMed: 18403594
Flow Cyt 1/50. PubMed: 20361950

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

WB 1/1000 - 1/5000. Detects a band of approximately 110-120 kDa (predicted molecular weight: 88 kDa). 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000 when using chemiluminescent substrates. Note: EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection.


  • Function
    In association with DPP4 is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers.
  • Tissue specificity
    Fibroblast specific.
  • Sequence similarities
    Belongs to the peptidase S9B family.
  • Post-translational
    The N-terminus may be blocked.
  • Cellular localization
    Cell membrane. Cell projection > lamellipodium membrane. Cell projection > invadopodium membrane. Found in cell surface lamellipodia, invadopodia and on shed vesicles. Colocalized with DPP4 in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with DPP4 on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma.
  • Information by UniProt
  • Database links
  • Alternative names
    • 170 kDa melanoma membrane bound gelatinase antibody
    • 170 kDa melanoma membrane-bound gelatinase antibody
    • DPPIV antibody
    • FAP antibody
    • FAPA antibody
    • Fibroblast activation protein alpha antibody
    • Integral membrane serine protease antibody
    • SEPR_HUMAN antibody
    • Seprase antibody
    see all


  • All lanes : Anti-Fibroblast activation protein, alpha antibody (ab28244) at 1/1000 dilution

    Lane 1 : Recombinant Human FAPalpha at 0.08 µg
    Lane 2 : Recombinant Human FAPalpha at 0.04 µg
    Lane 3 : Recombinant Human FAPalpha at 0.02 µg
    Lane 4 : Recombinant Human FAPalpha at 0.01 µg
    Lane 5 : Recombinant Human FAPalpha at 0.005 µg

    Predicted band size: 88 kDa

  • ICC/IF image of ab28244 stained MEF1 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28244, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:
  • Boulding T  et al. LSD1 activation promotes inducible EMT programs and modulates the tumour microenvironment in breast cancer. Sci Rep 8:73 (2018). Read more (PubMed: 29311580) »
  • Yang Z  et al. Dicer reprograms stromal fibroblasts to a pro-inflammatory and tumor-promoting phenotype in ovarian cancer. Cancer Lett 415:20-29 (2018). WB, IF . Read more (PubMed: 29199004) »

See all 17 Publications for this product

Customer reviews and Q&As

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Mouse Tissue sections (Mouse breast cancer)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris ph9 / citrate pH6
Yes - Tween 20 (0.1%)
Mouse breast cancer
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: rt°C

Abcam user community

Verified customer

Submitted Aug 10 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Mouse Tissue lysate - whole (Mouse breast tumors)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
50 µg
Mouse breast tumors
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Aug 10 2018

Thank you for calling us and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

As we discussed on the ...

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Thank you for contacting Abcam.

I would recommend using ab28244 or ab28246 as each of these is guaranteed to work in IF on rat tissues under our Abpromise.

The immunogen for ab28244 Fibroblast activation protein, alpha antibody st...

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Thank you for contacting Abcam.
We do not know of any publications which have used this product in IHC-P in mouse tissues.
A good starting point for determining the optimal concentration is to use the dilution used in the earlier referenced pa...

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Thank you for contacting us.

This product was used in IHC-P in the following publication: Bhati R et al. Molecular characterization of human breast tumor vascular cells. Am J Pathol 172:1381-90 (2008). IHC-P; Human. (PubMed: 18403594) (http:/...

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Thank you for your enquiry and your interest in our products.

The tested applications and the species are normally listed on the on-line product datasheets. Generally, we test our antibodies in human, mouse and rat samples - very few antibodi...

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Thank you for contacting Abcam regarding ab28244. Unfortunately the immunogen information for ab28244 is considered proprietary and I do not have access to that information. Please accept my apologies for not being able to provide you w...

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Thank you for your reply. I can confirm that only ab28244 (Anti-Fibroblast activation protein, alpha antibody - Stalk region) is tested and guaranteed for flow cytometry and I therefore recommend this antibody for your experiments. Please...

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Thank you for contacting us. I can confirm that ab28244 is tested and guaranteed for FACS and IHC-P in mouse, human and rat by our Abpromise. and therefore should be suitable to isolate cells that express FAP. This antibody recognizes t...

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