Product nameAnti-Fibroblast activation protein, alpha antibody
See all Fibroblast activation protein, alpha primary antibodies
DescriptionRabbit polyclonal to Fibroblast activation protein, alpha
SpecificityThis antibody recognizes the Stalk Region of both long isoforms of Fibroblast activation protein, alpha but does not recognize other DPP family members.
Tested applicationsSuitable for: ICC/IF, IHC-P, Flow Cyt, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Fibroblast activation protein, alpha. The exact sequence is proprietary.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.05% Sodium azide
Constituent: 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab28244 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 10 µg/ml.|
|IHC-P||Use at an assay dependent concentration. PubMed: 18403594|
|Flow Cyt||1/50. PubMed: 20361950
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/1000 - 1/5000. Detects a band of approximately 110-120 kDa (predicted molecular weight: 88 kDa). 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000 when using chemiluminescent substrates. Note: EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection.|
FunctionIn association with DPP4 is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers.
Tissue specificityFibroblast specific.
Sequence similaritiesBelongs to the peptidase S9B family.
The N-terminus may be blocked.
Cellular localizationCell membrane. Cell projection > lamellipodium membrane. Cell projection > invadopodium membrane. Found in cell surface lamellipodia, invadopodia and on shed vesicles. Colocalized with DPP4 in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with DPP4 on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma.
- Information by UniProt
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All lanes : Anti-Fibroblast activation protein, alpha antibody (ab28244) at 1/1000 dilution
Lane 1 : Recombinant Human FAPalpha at 0.08 µg
Lane 2 : Recombinant Human FAPalpha at 0.04 µg
Lane 3 : Recombinant Human FAPalpha at 0.02 µg
Lane 4 : Recombinant Human FAPalpha at 0.01 µg
Lane 5 : Recombinant Human FAPalpha at 0.005 µg
Predicted band size: 88 kDa
ICC/IF image of ab28244 stained MEF1 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28244, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Gao Q et al. Heterotypic CAF-tumor spheroids promote early peritoneal metastatis of ovarian cancer. J Exp Med 216:688-703 (2019). Read more (PubMed: 30710055) »
- Sandberg TP et al. Increased expression of cancer-associated fibroblast markers at the invasive front and its association with tumor-stroma ratio in colorectal cancer. BMC Cancer 19:284 (2019). Read more (PubMed: 30922247) »