Anti-Fibroblast activation protein, alpha antibody (ab28244)
Key features and details
- Rabbit polyclonal to Fibroblast activation protein, alpha
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Fibroblast activation protein, alpha antibody
See all Fibroblast activation protein, alpha primary antibodies -
Description
Rabbit polyclonal to Fibroblast activation protein, alpha -
Host species
Rabbit -
Specificity
This antibody recognizes the Stalk Region of both long isoforms of Fibroblast activation protein, alpha but does not recognize other DPP family members. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Recombinant fragment -
Immunogen
Synthetic peptide within Human Fibroblast activation protein, alpha. The exact sequence is proprietary.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 50% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab28244 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000 - 1/5000. Detects a band of approximately 110-120 kDa (predicted molecular weight: 88 kDa). 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000 when using chemiluminescent substrates. Note: EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection.
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Notes |
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WB
1/1000 - 1/5000. Detects a band of approximately 110-120 kDa (predicted molecular weight: 88 kDa). 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000 when using chemiluminescent substrates. Note: EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection. |
Target
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Function
In association with DPP4 is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers. -
Tissue specificity
Fibroblast specific. -
Sequence similarities
Belongs to the peptidase S9B family. -
Post-translational
modificationsN-glycosylated.
The N-terminus may be blocked. -
Cellular localization
Cell membrane. Cell projection > lamellipodium membrane. Cell projection > invadopodium membrane. Found in cell surface lamellipodia, invadopodia and on shed vesicles. Colocalized with DPP4 in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with DPP4 on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma. - Information by UniProt
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Alternative names
- 170 kDa melanoma membrane bound gelatinase antibody
- 170 kDa melanoma membrane-bound gelatinase antibody
- DPPIV antibody
see all
Images
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All lanes : Anti-Fibroblast activation protein, alpha antibody (ab28244) at 1/1000 dilution
Lane 1 : Recombinant Human FAPalpha at 0.08 µg
Lane 2 : Recombinant Human FAPalpha at 0.04 µg
Lane 3 : Recombinant Human FAPalpha at 0.02 µg
Lane 4 : Recombinant Human FAPalpha at 0.01 µg
Lane 5 : Recombinant Human FAPalpha at 0.005 µg
Predicted band size: 88 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (57)
ab28244 has been referenced in 57 publications.
- Chen S et al. Epigallocatechin‑3‑gallate hinders metabolic coupling to suppress colorectal cancer malignancy through targeting aerobic glycolysis in cancer‑associated fibroblasts. Int J Oncol 60:N/A (2022). PubMed: 35029285
- Wu T et al. Targeting HIC1/TGF-β axis-shaped prostate cancer microenvironment restrains its progression. Cell Death Dis 13:624 (2022). PubMed: 35853880
- Kim JY et al. Oral Squamous Cell Carcinoma-Derived ANGPTL3 Induces Cancer Associated Fibroblastic Phenotypes in Surrounding Fibroblasts. Asian Pac J Cancer Prev 23:4315-4322 (2022). PubMed: 36580015
- Kato R et al. Nintedanib promotes antitumour immunity and shows antitumour activity in combination with PD-1 blockade in mice: potential role of cancer-associated fibroblasts. Br J Cancer 124:914-924 (2021). PubMed: 33299131
- Pizzurro GA et al. 3D Model of the Early Melanoma Microenvironment Captures Macrophage Transition into a Tumor-Promoting Phenotype. Cancers (Basel) 13:N/A (2021). PubMed: 34572807