Anti-Fibroblast activation protein, alpha antibody (ab53066)
Key features and details
- Rabbit polyclonal to Fibroblast activation protein, alpha
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-Fibroblast activation protein, alpha antibody
See all Fibroblast activation protein, alpha primary antibodies -
Description
Rabbit polyclonal to Fibroblast activation protein, alpha -
Host species
Rabbit -
Specificity
ab53066 detects endogenous levels of total Fibroblast activation protein, alpha. Please note the immunogen used to produce this product has 92% homology with Mouse DPPIV/CD26. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
-
Positive control
- Human breast carcinoma tissue and LOVO cell extracts.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Store at -20°C. Stable for 12 months at -20°C -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
ab53066 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53066 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (4) |
1/500 - 1/1000. Detects a band of approximately 90 kDa.
Please see WB protocol details in the image legend. |
IHC-P | (5) |
1/50 - 1/100.
Antigen retrieval: Microwave method - put the slice into 10 mmol/L citrate buffer (pH 6.0), microwave high temperature for 5 minutes, and then medium temperature for 15 minutes. Primary antibody incubation: 1 hour at 37°C Secondary antibody: Poly-HRP-Anti Mouse/Rabbit IgG, 50 µL for 30 minutes. DAB reagent. |
Notes |
---|
WB
1/500 - 1/1000. Detects a band of approximately 90 kDa. Please see WB protocol details in the image legend. |
IHC-P
1/50 - 1/100. Antigen retrieval: Microwave method - put the slice into 10 mmol/L citrate buffer (pH 6.0), microwave high temperature for 5 minutes, and then medium temperature for 15 minutes. Primary antibody incubation: 1 hour at 37°C Secondary antibody: Poly-HRP-Anti Mouse/Rabbit IgG, 50 µL for 30 minutes. DAB reagent. |
Target
-
Function
In association with DPP4 is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers. -
Tissue specificity
Fibroblast specific. -
Sequence similarities
Belongs to the peptidase S9B family. -
Post-translational
modificationsN-glycosylated.
The N-terminus may be blocked. -
Cellular localization
Cell membrane. Cell projection > lamellipodium membrane. Cell projection > invadopodium membrane. Found in cell surface lamellipodia, invadopodia and on shed vesicles. Colocalized with DPP4 in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with DPP4 on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma. - Information by UniProt
-
Database links
- Entrez Gene: 2191 Human
- Omim: 600403 Human
- SwissProt: Q12884 Human
- Unigene: 654370 Human
-
Alternative names
- 170 kDa melanoma membrane bound gelatinase antibody
- 170 kDa melanoma membrane-bound gelatinase antibody
- DPPIV antibody
see all
Images
-
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue sections labeling Fibroblast activation protein, alpha with ab53066 (without peptide-right and with peptide- left).
-
All lanes : Anti-Fibroblast activation protein, alpha antibody (ab53066) at 1/500 dilution
Lane 1 : LOVO cell extract
Lane 2 : LOVO cell extract at 40 µg with immunising peptide
Observed band size: 90 kDa why is the actual band size different from the predicted?Blocking buffer: 5% (w/v) non-fat dry milk in TBST.
Primary antibody dilution buffer: 5%(w/v)non-fat dried milk,0.1%(v/v), Tween-20 in TBST.
Secondary antibody dilution buffer: 5%(w/v)non-fat dried milk,0.1%(v/v),Tween-20 in TBST.
12% SDS gel. Nitrocellulose membrane.
Blocking: Room temperature for 2 hours or overnight at 4°C. Then wash 3x for 5 minutes with 0.05% blocking buffer.
Primary antibody incubation: diluted in TBST at 1/500. Incubate overnight with 4 degrees shaking. Then, in 0.05% TBST, wash membrane 3-4 times for 10min.
Secondary antibody incubation: diluted in TBST at 1/2000. Incubate 37°C for 1 hour. Then, in 0.05% TBST, wash membrane 3-4 times for 10min.
ECL development.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (116)
ab53066 has been referenced in 116 publications.
- Thongchot S et al. Novel CSF1R-positive tenosynovial giant cell tumor cell lines and their pexidartinib (PLX3397) and sotuletinib (BLZ945)-induced apoptosis. Hum Cell 36:456-467 (2023). PubMed: 36456782
- Ambrosetti D et al. Cancer-associated fibroblasts in renal cell carcinoma: implication in prognosis and resistance to anti-angiogenic therapy. BJU Int 129:80-92 (2022). PubMed: 34107167
- Röhrich M et al. Fibroblast Activation Protein-Specific PET/CT Imaging in Fibrotic Interstitial Lung Diseases and Lung Cancer: A Translational Exploratory Study. J Nucl Med 63:127-133 (2022). PubMed: 34272325
- Liu Y et al. Fibroblast activation protein targeted therapy using [177Lu]FAPI-46 compared with [225Ac]FAPI-46 in a pancreatic cancer model. Eur J Nucl Med Mol Imaging 49:871-880 (2022). PubMed: 34537893
- Wei Y et al. [18F]AlF-NOTA-FAPI-04 PET/CT uptake in metastatic lesions on PET/CT imaging might distinguish different pathological types of lung cancer. Eur J Nucl Med Mol Imaging 49:1671-1681 (2022). PubMed: 34870727