Recombinant
RabMAb

Recombinant Anti-Fibroblast activation protein, alpha antibody [SP325] (ab227703)

Overview

  • Product name

    Anti-Fibroblast activation protein, alpha antibody [SP325]
    See all Fibroblast activation protein, alpha primary antibodies
  • Description

    Rabbit monoclonal [SP325] to Fibroblast activation protein, alpha
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Synthetic peptide within Human Fibroblast activation protein, alpha aa 300-400 (internal sequence). The exact sequence is proprietary.
    Database link: Q12884

  • Positive control

    • IHC-P: Human melanoma, colon adenocarcinoma, ovarian adenocarcinoma, pancreatic adenocarcinoma, lung squamous cell carcinoma, skin squamous cell carcinoma and cervical squamous cell carcinoma tissues.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.6
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from TCS by protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    SP325
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227703 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.

Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.

Primary antibody incubation for 10 minutes at room temperature.

Target

  • Function

    In association with DPP4 is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers.
  • Tissue specificity

    Fibroblast specific.
  • Sequence similarities

    Belongs to the peptidase S9B family.
  • Post-translational
    modifications

    N-glycosylated.
    The N-terminus may be blocked.
  • Cellular localization

    Cell membrane. Cell projection > lamellipodium membrane. Cell projection > invadopodium membrane. Found in cell surface lamellipodia, invadopodia and on shed vesicles. Colocalized with DPP4 in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with DPP4 on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma.
  • Information by UniProt
  • Database links

  • Alternative names

    • 170 kDa melanoma membrane bound gelatinase antibody
    • 170 kDa melanoma membrane-bound gelatinase antibody
    • DPPIV antibody
    • FAP antibody
    • FAPA antibody
    • Fibroblast activation protein alpha antibody
    • Integral membrane serine protease antibody
    • SEPR_HUMAN antibody
    • Seprase antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue sections labeling Fibroblast activation protein, alpha with ab227703 at 1/100 dilution (1.76 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the stroma in the human colon carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab227703 for 10 mins at room temperature.

  • Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for Fibroblast activation protein, alpha using ab227703 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for Fibroblast activation protein, alpha using ab227703 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for Fibroblast activation protein, alpha using ab227703 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for Fibroblast activation protein, alpha using ab227703 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue stained for Fibroblast activation protein, alpha using ab227703 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human melanoma tissue stained for Fibroblast activation protein, alpha using ab227703 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human cervical squamous cell carcinoma tissue stained for Fibroblast activation protein, alpha using ab227703 at 1/100 dilution in immunohistochemical analysis.

References

This product has been referenced in:

  • Liu J  et al. Stromal fibroblast activation protein alpha promotes gastric cancer progression via epithelial-mesenchymal transition through Wnt/ ß-catenin pathway. BMC Cancer 18:1099 (2018). Read more (PubMed: 30419872) »
See 1 Publication for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (mouse tumor tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate ph 6 or ph 9
Permeabilization
No
Specification
mouse tumor tissue
Blocking step
Rodent M blocker (Biocare) as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Oct 03 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Human neuroblastoma xenograft in mouse)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: ab93680
Permeabilization
No
Specification
Human neuroblastoma xenograft in mouse
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Dec 15 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mouse ovarian cancer tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA
Permeabilization
No
Specification
Mouse ovarian cancer tissue
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Formaldehyde

Dr. Lixin Zhang

Verified customer

Submitted Aug 02 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Pancreatic Adenocarcinoma (PDAC))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Ventana CC1 Solution
Specification
Pancreatic Adenocarcinoma (PDAC)
Blocking step
(agent) for 12 minute(s) · Concentration: 10% · Temperature: 37°C
Fixative
10% NBF

Abcam user community

Verified customer

Submitted Jul 31 2018

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