RecombinantRabMAb

Recombinant Anti-Fibroblast activation protein, alpha antibody [SP325] - BSA and Azide free (ab240989)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [SP325] - BSA and Azide free (ab240989)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [SP325] - BSA and Azide free (ab240989)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [SP325] - BSA and Azide free (ab240989)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [SP325] - BSA and Azide free (ab240989)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [SP325] - BSA and Azide free (ab240989)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [SP325] - BSA and Azide free (ab240989)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [SP325] - BSA and Azide free (ab240989)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [SP325] - BSA and Azide free (ab240989)
  • Anti-Fibroblast activation protein, alpha antibody [SP325] - BSA and Azide free (ab240989)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP325] to Fibroblast activation protein, alpha - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Fibroblast activation protein, alpha antibody [SP325] - BSA and Azide free
    See all Fibroblast activation protein, alpha primary antibodies

  • Description

    Rabbit monoclonal [SP325] to Fibroblast activation protein, alpha - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Cow

  • Immunogen

    Synthetic peptide within Human Fibroblast activation protein, alpha aa 300-400 (internal sequence). The exact sequence is proprietary.
    Database link: Q12884

  • Positive control

    • IHC-P: Human melanoma, colon adenocarcinoma, ovarian adenocarcinoma, pancreatic adenocarcinoma, lung squamous cell carcinoma, skin squamous cell carcinoma and cervical squamous cell carcinoma tissues. WB: HFF-1 cell lysate.

  • General notes

    FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    ab240989 is the carrier-free version of ab227703. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240989 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab240989 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.

Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.Primary antibody incubation for 10 minutes at room temperature.

 
WB Use at an assay dependent concentration. Predicted molecular weight: 88 kDa.

Target

  • Function

    In association with DPP4 is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers.

  • Tissue specificity

    Fibroblast specific.

  • Sequence similarities

    Belongs to the peptidase S9B family.

  • Post-translational
    modifications

    N-glycosylated.
    The N-terminus may be blocked.

  • Cellular localization

    Cell membrane. Cell projection > lamellipodium membrane. Cell projection > invadopodium membrane. Found in cell surface lamellipodia, invadopodia and on shed vesicles. Colocalized with DPP4 in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with DPP4 on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 170 kDa melanoma membrane bound gelatinase antibody
    • 170 kDa melanoma membrane-bound gelatinase antibody
    • DPPIV antibody
    • FAP antibody
    • FAPA antibody
    • Fibroblast activation protein alpha antibody
    • Integral membrane serine protease antibody
    • SEPR_HUMAN antibody
    • Seprase antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [SP325] - BSA and Azide free (ab240989)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [SP325] - BSA and Azide free (ab240989)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue sections labeling Fibroblast activation protein, alpha with ab227703 at 1/100 dilution (1.76 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the stroma in the human colon carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab227703 for 10 mins at room temperature.

    This image was generated using ab227703, the same clone, but with a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [SP325] - BSA and Azide free (ab240989)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [SP325] - BSA and Azide free (ab240989)

    Formalin-fixed, paraffin-embedded human cervical squamous cell carcinoma tissue stained for Fibroblast activation protein, alpha using ab227703 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227703)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [SP325] - BSA and Azide free (ab240989)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [SP325] - BSA and Azide free (ab240989)

    Formalin-fixed, paraffin-embedded human melanoma tissue stained for Fibroblast activation protein, alpha using ab227703 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227703)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [SP325] - BSA and Azide free (ab240989)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [SP325] - BSA and Azide free (ab240989)

    Formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue stained for Fibroblast activation protein, alpha using ab227703 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227703)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [SP325] - BSA and Azide free (ab240989)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [SP325] - BSA and Azide free (ab240989)

    Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for Fibroblast activation protein, alpha using ab227703 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227703)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [SP325] - BSA and Azide free (ab240989)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [SP325] - BSA and Azide free (ab240989)

    Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for Fibroblast activation protein, alpha using ab227703 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227703)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [SP325] - BSA and Azide free (ab240989)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [SP325] - BSA and Azide free (ab240989)

    Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for Fibroblast activation protein, alpha using ab227703 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227703)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [SP325] - BSA and Azide free (ab240989)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibroblast activation protein, alpha antibody [SP325] - BSA and Azide free (ab240989)

    Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for Fibroblast activation protein, alpha using ab227703 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227703)
  • Anti-Fibroblast activation protein, alpha antibody [SP325] - BSA and Azide free (ab240989)
    Anti-Fibroblast activation protein, alpha antibody [SP325] - BSA and Azide free (ab240989)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab240989? Please let us know so that we can cite the reference in this datasheet.

ab240989 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Immunocytochemistry/ Immunofluorescence abreview for Anti-Fibroblast activation protein, alpha antibody [SP325] - BSA and Azide free

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (breast cancer cell line in vivo (EMT6))
Permeabilization
No
Specification
breast cancer cell line in vivo (EMT6)
Blocking step
Akoya CODEX Blocking Buffer as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Victoria Koch

Verified customer

Submitted Oct 21 2020

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com