Product nameAnti-Fibromodulin antibody [EPR23029-174] - BSA and Azide free
See all Fibromodulin primary antibodies
DescriptionRabbit monoclonal [EPR23029-174] to Fibromodulin - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment within Human Fibromodulin aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: Q06828
- WB: Recombinant Human Fibromodulin/FMOD protein, Human articular cartilage, Human uterus, Mouse articular cartilage, Rat articular cartilage, Human uterus and Human uterus treated with PNGase F lysates.
ab269866 is the carrier-free version of ab267465. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab269866 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.??
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab269866 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 43 kDa.|
FunctionAffects the rate of fibrils formation. May have a primary role in collagen fibrillogenesis.
Sequence similaritiesBelongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class II subfamily.
Contains 11 LRR (leucine-rich) repeats.
Contains 1 LRRNT domain.
modificationsBinds keratan sulfate chains.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- Collagen binding 59 kDa protein antibody
- Collagen-binding 59 kDa protein antibody
- Fibromodulin antibody
All lanes : Anti-Fibromodulin antibody [EPR23029-174] (ab267465) at 1/1000 dilution
Lane 1 : Human articular cartilage lysate
Lane 2 : Human uterus lysate
Lane 3 : Human skin lysate
Lane 4 : HuT-78 (human sezary syndrome cutaneous t lymphocyte) whole cell lysate
Lane 5 : Mouse articular cartilage lysate
Lane 6 : Rat articular cartilage lysate
Lysates/proteins at 20 µg per lane.
Lanes 1-4 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Lanes 5-6 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution
Predicted band size: 43 kDa
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 12623288, 8895216)
Negative control: Hut-78 (PMID: 15741214)
Exposure time: Lane 1 second; Lanes 2-6:3 secondsThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab267465).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab269866 has not yet been referenced specifically in any publications.