Recombinant Anti-Fibronectin antibody [10/Fibronectin] (ab281575)
![Western blot - Anti-Fibronectin antibody [10/Fibronectin] (ab281575)](https://www.abcam.com/ps/products/281/ab281575/Images/ab281575-471156-anti-fibronectin-antibody-10fibronectin-western-blot-wild-type-hap1.jpg "Western blot - Anti-Fibronectin antibody [10/Fibronectin] (ab281575)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [10/Fibronectin] to Fibronectin
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Fibronectin antibody [10/Fibronectin]
See all Fibronectin primary antibodies -
Description
Mouse monoclonal [10/Fibronectin] to Fibronectin -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: ICC,IHC-Fr or IP -
Species reactivity
Reacts with: Human -
Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- WB: Wild type HAP1, HepG2, Human plasma, U-87 MG lysates. IHC-P: Human colon cancer, Human ovarian cancer, Human testis tissues.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
10/Fibronectin -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab281575 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500.
|
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| IHC-P |
1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
|
WB
1/500. |
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IHC-P
1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. -
Tissue specificity
Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine. -
Involvement in disease
Glomerulopathy with fibronectin deposits 2 -
Sequence similarities
Contains 12 fibronectin type-I domains.
Contains 2 fibronectin type-II domains.
Contains 16 fibronectin type-III domains. -
Developmental stage
Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age. -
Post-translational
modificationsSulfated.
It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
Phosphorylated by FAM20C in the extracellular medium.
Proteolytic processing produces the C-terminal NC1 peptide, anastellin. -
Cellular localization
Secreted, extracellular space, extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 2335 Human
- Omim: 135600 Human
- SwissProt: P02751 Human
- Unigene: 203717 Human
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Alternative names
- CIG antibody
- Cold insoluble globulin antibody
- Cold-insoluble globulin antibody
see all
Images
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Western blot - Anti-Fibronectin antibody [10/Fibronectin] (ab281575)All lanes : Anti-Fibronectin antibody [10/Fibronectin] (ab281575) at 1/500 dilution
Lane 1 : Wild type HAP1(human chronic myelogenous leukemia near-haploid cell line), whole cell lysate
Lane 2 : Fibronectin knockout HAP1(human chronic myelogenous leukemia near-haploid cell line), whole cell lysate
Lane 3 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (IRDye® 800CW) (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) (ab216777) at 1/10000 dilution
Observed band size: 285 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS
The expression molecular weight observed is consistent with what has been described in the literature (PMID:28367057).
Lanes 1-3: Merged signal (red and green). Green - ab281575 observed at 285kDa. Red - loading control ab52901(Rabbit monoclonal [EP1331Y] to beta Tubulin) observed at 50 kDa.
Lanes 1-2: ab281575 Anti-Fibronectin antibody was shown to react with Fibronectin in HAP1 cells in Western blot. Loss of signal was observed when Fibronectin knockout sample was used. Wild-type and Fibronectin knockout samples were subjected to SDS-PAGE.
ab281575 and Anti-beta Tubulin antibody [EP1331Y] - Loading Control (ab52901) were incubated at 4? overnight at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680CW) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800RD) preadsorbed (ab216772) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging. -
![Western blot - Anti-Fibronectin antibody [10/Fibronectin] (ab281575)](https://www.abcam.com/ps/products/281/ab281575/Images/ab281575-471158-anti-fibronectin-antibody-10fibronectin-western-blot-human-plasma-lysate-20-ug.jpg)
Western blot - Anti-Fibronectin antibody [10/Fibronectin] (ab281575)All lanes : Anti-Fibronectin antibody [10/Fibronectin] (ab281575) at 1/1000 dilution
Lane 1 : Human plasma lysate at 20 µg
Lane 2 : U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate at 40 µg
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Observed band size: 285 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Samples are non-boiled as boiling may cause protein aggregates.
The expression molecular weight observed is consistent with what has been described in the literature (PMID:28367057).
Exposure time: lane 1: 3 secondslane 2: 37 seconds
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [10/Fibronectin] (ab281575)](https://www.abcam.com/ps/products/281/ab281575/Images/ab281575-3-anti-fibronectin-antibody-10fibronectin-immunohistochemistry-human-colon-cancer.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [10/Fibronectin] (ab281575)Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling Fibronectin with abab281575 at 1/2000 (0.551 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining in stromal cells of human colon cancer.The section was incubated with ab281575 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND™ RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) .
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [10/Fibronectin] (ab281575)](https://www.abcam.com/ps/products/281/ab281575/Images/ab281575-4-anti-fibronectin-antibody-10fibronectin-immunohistochemistry-human-ovarian-cancer.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [10/Fibronectin] (ab281575)Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue labeling Fibronectin with ab281575 at 1/2000 (0.551 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) . Positive staining in stromal cells of human ovarian cancer.The section was incubated with ab281575 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) .
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [10/Fibronectin] (ab281575)](https://www.abcam.com/ps/products/281/ab281575/Images/ab281575-5-anti-fibronectin-antibody-10fibronectin-immunohistochemistry-human-testis.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [10/Fibronectin] (ab281575)Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Fibronectin with ab281575 at 1/2000 (0.551 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) . Positive staining in stromal cells of human testis.The section was incubated with ab281575 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) .
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
-
![Anti-Fibronectin antibody [10/Fibronectin] (ab281575)](https://www.abcam.com/ps/products/281/ab281575/Images/ab281575-6-Benefits-of-Recombinant-antibodies.jpg)
Anti-Fibronectin antibody [10/Fibronectin] (ab281575)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab281575 has not yet been referenced specifically in any publications.